{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T18:20:46Z","timestamp":1700245246518},"reference-count":75,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3663,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In leech, the central annulus of each midbody segment possesses seven pairs of sensilla, which are mixed clusters of primary peripheral sensory neurons that extend their axons into the CNS where they segregate into distinct fascicles. Pathway selection by individual afferent growth cones of sensillar neurons was examined by double labeling using intracellular dye\u2010filling with anitobody labeling in early <jats:italic>Hirudo medicinalis<\/jats:italic> embryos. The monoclonal antibody Lan3\u20132 was used because sensillar neuronal tracts are specifically labeled by this antibody. Examining 68 individually filled neurons we found that sensillar neuron growth cones bifurcate within the CNS, that they project long filopodia capable to sampling the local environment, and that all of them appeared to choose a single particular CNS fascicle without apparent retraction or realignment of growth cones. Furthermore, each side of the bifurcating afferent growth cones always chose the same fascicle, implying a specific choice of a distinct labeled pathway. By dye\u2010filling individual central neurons (P\u2010cells), we show that there are centrally projecting axons present at the time sensillar afferents enter the ganglionic primordia and select a particular fascicle, and we confirm that at least the dorsal peripheral nerve is likely to be pioneered by central neurons, not by the peripheral afferent. In the sensillum studied here, we sound examples of sensory neurons extending axons into one of all the avilable fascicles. Thus, an individual embryonic sensillum possesses a heterogeneous population of afferents with respect to the central fascicle chosen. This is consistent with the idea that segregation into distinct axon fascicles may be based upon functional differences between individual afferent neurons. Our findings argue strongly in favor of specific pathway selection by afferents in this system and are consistent with previous suggestions that there exists a hierarchy of cues, including surface glycoconjugates that mediate navigation of the sensillar growth cones and the fasciculation of their axons. 1994 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/neu.480251002","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T03:42:16Z","timestamp":1104550936000},"page":"1187-1199","source":"Crossref","is-referenced-by-count":14,"title":["Specific pathway selection by the early projections of individual peripheral sensory neurons in the embryonic medicinal leech"],"prefix":"10.1002","volume":"25","author":[{"given":"John","family":"Jellies","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen","family":"Johansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen","family":"Johansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490270306"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/346855a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-12-03518.1986"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/260054a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.218.4577.1082"},{"key":"e_1_2_1_7_1","first-page":"265","volume-title":"The Nerve Growth Cone","author":"Bentley D.","year":"1992"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.223.4635.493"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"4165","DOI":"10.1523\/JNEUROSCI.07-12-04165.1987","article-title":"The guidance of axons from transplanted neurons through aneural Drosophila wings","volume":"7","author":"Blair S. 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