{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:58:27Z","timestamp":1762160307966},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3663,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isolated adult frog dorsal root ganglion neurons survive <jats:italic>in vitro<\/jats:italic> in a defined medium for more than 4 weeks and extend processes. When co\u2010cultured with a 1\u2010mm piece of peripheral nerve the average tottal process lenght per neuron was 10 times longer than that of control neurons by 8 days, and the processes had a significantly different morphology from that of control neurons. This influence on process length increased with increasing time of nerve denervation length increased with increasing time of nerve denervation prior to co\u2010culturing. These results suggest the release of a neurotrophic factor\/s from the cells of the peripheral nerve. The neurotropic influence was completely blocked by antibodies against mouse nerve growth factor (NGF). Although NGF increased the average process length by twofold over control neurons, its influence never reached that of the nerve\u2010released factor, and the NGF\u2010induced processes had a distinctly different morphology. The frog nerve\u2010released factor promoted process outgrowth from E11 chick sympathetic ganglia, although the process number, length, and their fasciculation differed greatly from those induced by NGF. These results suggest that the nerve\u2010released factor\/s are immunologically and functionally related to NGF but have not estabished whether a single factor or an aggregate of several secreted molecules are responsible. This article presents a new preparation in which the varied influences of different neurotrophic factors can be studied in great detail on large populations of isolated adult vertebrte neurons and sets the stage for the characterization and isolation of the frog peripheral nerve neurotrophic factor, as well as examining the influence of this facor on neuronal morphology and its ability to direct process outgrowth. 1994 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/neu.480251008","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T03:42:16Z","timestamp":1104550936000},"page":"1267-1282","source":"Crossref","is-referenced-by-count":21,"title":["Neutrophic influence of denervated sciatic nerve of on adult dorsal root ganglion neurons"],"prefix":"10.1002","volume":"25","author":[{"given":"Damien P.","family":"Kuffler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Megwinoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90265-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/291413a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb04835.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb05410.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.2.1199"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90287-A"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/319497a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-10-02662.1985"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90213-W"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp017936"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5454"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90090-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1991.tb00031.x"},{"key":"e_1_2_1_15_1","first-page":"446","article-title":"Reduction of CNTF levels in Schwann cells in degenerating sciatic nerve","volume":"17","author":"Friedman B. Ip. N. Y.","year":"1991","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.2.455"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90309-6"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903120313"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.9.5895"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90341-8"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90180-8"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.01-01-00060.1981"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/344339a0"},{"key":"e_1_2_1_24_1","first-page":"466","article-title":"Distribution of CNTF and its receptor in the CNS and PNS","volume":"17","author":"Ip N. Y.","year":"1991","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7192014"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/314751a0"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(86)90012-3"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.11.3513"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902490106"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902810307"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903020405"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1152\/physrev.1968.48.3.534","article-title":"Nerve growth factor","volume":"48","author":"Levi\u2010Montalcini R.","year":"1968","journal-title":"Physiol. Rev."},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/341149a0"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-07-02394.1988"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321006"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.1.45"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-07-02853.1993"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490310124"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440050105"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902300110"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90141-X"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90203-R"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90284-K"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90102-0"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/358502a0"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.118.1.139"},{"key":"e_1_2_1_47_1","first-page":"446","article-title":"Down\u2010regulation of CNTF mRNA during peripheral nerve degeneration","volume":"17","author":"Seniuk N.","year":"1991","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(90)90203-U"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/342920a0"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(92)90126-H"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1980.60.4.1284"},{"key":"e_1_2_1_52_1","unstructured":"Vale Y. Kuffler D. P. andSantiageo C.(1993).Factores neurotroficos que promueven la regeneracion de los nervios en Rana pipiens. 13th ACS Technical Meeting Abstr. 13:14."},{"key":"e_1_2_1_53_1","first-page":"323","article-title":"Nerve growth factor induces a dosedependent and long\u2010lasting increase of choline acetyltransferase activity in the septal area and hippocampus of uninjured rat","volume":"50","author":"Vantini G.","year":"1990","journal-title":"Acta Neurobiol. Exp. (Warsaz)."},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-11-03703.1987"}],"container-title":["Journal of Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fneu.480251008","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/neu.480251008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T16:19:14Z","timestamp":1698164354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/neu.480251008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":53,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1002\/neu.480251008"],"URL":"https:\/\/doi.org\/10.1002\/neu.480251008","archive":["Portico"],"relation":{},"ISSN":["0022-3034","1097-4695"],"issn-type":[{"value":"0022-3034","type":"print"},{"value":"1097-4695","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}