{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:34:58Z","timestamp":1791153298790,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3359,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of homozygotic Wnt\u20101<jats:sup>\u2212\/\u2212<\/jats:sup> mutations on the development of ocular motoneurons was examined with the lipophilic dye DiI and compared to control and phenotypic wild\u2010type mouse embryos. A piece of DiI\u2010soaked filter paper was inserted into the orbit, the midbrain, or rhombomere 5 of the hindbrain in six paraformaldehydefixed litters (10.5, 12.5, and 14.5 days postcoitum) containing Wnt\u20101, Wnt<jats:sup>+\/\u2212<\/jats:sup>, and Wnt\u20101<jats:sup>+\/+<\/jats:sup> individuals and three control litters. We labeled all ocular motoneurons retrogradely and all relevant nerves anterogradely in all control and phenotypic wild\u2010type animals. In all phenotypically identified Wnt\u20101<jats:sup>\u2212\/\u2212<\/jats:sup> mutants we could always label the abducens nerve and motoneurons and the optic fibers to the thalamus, but we were unable to lable oculomotor or trochlear nerves or motoneurons. In addition to Wnt\u20101 knockout mutants, we also labeled mice from the WZT9B transgenic line carrying a <jats:italic>lacZ<\/jats:italic> reporter gene driven by the Wnt\u20101 gene enhancer. In these embryos we tested for co\u2010localization of Wnt\u20101 expression in biotinylated dextran amine\u2010labeled ocular motoneurons using a newly developed technique. In younger embryos we obtained evidence for co\u2010localization of the \u03b2\u2010galactosidase reaction product derived from <jats:italic>lacZ<\/jats:italic> gene activity in some retrogradely filled oculomotor motoneurons and adjacent to other oculomotor and the trochlear motoneurons. Acetylcholine esterase, a marker of early differentiating cholinergic neurons, showed a similar topology with respect to the lacZ reaction product. Thus, at least some future oculomotor motoneurons express Wnt\u20101, whereas others and the trochlear motoneurons caudal to the ventral midbrain expression of Wnt\u20101 may be exposed to the short range diffusion of the Wnt\u20101 gene product. Thus, the Wnt\u20101<jats:sup>\u2212\/\u2212<\/jats:sup> mutation precludes formation or survival of midbrain and anterior hindbrain neurons, including oculomotor and trochlear motoneurons. \u00a9 1995 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/neu.480270403","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T03:52:00Z","timestamp":1104551520000},"page":"457-469","source":"Crossref","is-referenced-by-count":54,"title":["Development of midbrain and anterior hindbrain ocular motoneurons in normal and Wnt\u20101 knockout mice"],"prefix":"10.1002","volume":"27","author":[{"given":"B.","family":"Fritzsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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