{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:40:51Z","timestamp":1791895251151,"version":"4.3.4"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["17K11858"],"award-info":[{"award-number":["17K11858"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007854","name":"School of Dentistry, Nihon University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007854","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropsychopharm Rep"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Orexin\u2010A is known to induce anti\u2010nociceptive effects in animal models of chronic pain. We have found that orexin\u2010A inhibits KCl loading\u2010induced increases in the intracellular calcium ion levels ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in C\u2010fiber\u2010like neurons of rats showing inflammatory nociceptive behavior. Here, we examined the effects of orexin\u2010A on the depolarization of C\u2010fiber\u2010like neurons derived from a rat model for another type of chronic pain, namely neuropathic pain. Thus, we analyzed the effects of orexin\u2010A on KCl\u2010induced increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in C\u2010fiber\u2010like neurons of rats with sciatic nerve ligation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paw withdrawal and threshold force in response to tactile stimuli were evaluated using von Frey filaments. Sham\u2010operated rats served as controls. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in neurons were visualized by calcium fluorescent probe. Changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> were assessed using relative fluorescence intensity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seven days after sciatic nerve ligation, paw withdrawal and threshold force for tactile stimuli were increased and reduced, respectively. KCl loading to neurons from either sciatic nerve\u2010ligated or control rats increased relative fluorescence intensity. The KCl\u2010induced increase in relative fluorescence intensity in sciatic nerve\u2010ligated, but not that of control, rats was inhibited by orexin\u2010A. The OX<jats:sub>1<\/jats:sub> and OX<jats:sub>2<\/jats:sub> receptor antagonist MK\u20104305 and OX<jats:sub>2<\/jats:sub> receptor antagonist EMPA, but not the OX<jats:sub>1<\/jats:sub> receptor antagonist SB\u00a0334867, each counteracted orexin\u2010A\u2010induced inhibition of KCl\u2010provoked increases in relative fluorescence intensity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present findings constitute neuropharmacological evidence that OX<jats:sub>2<\/jats:sub> but not OX<jats:sub>1<\/jats:sub> receptors mediate the inhibitory effects of orexin\u2010A on KCl\u2010induced increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in C\u2010fiber\u2010like neurons of rats showing hyperalgesia provoked by sciatic nerve ligation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/npr2.12094","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T09:03:55Z","timestamp":1576573435000},"page":"30-38","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["OX<sub>2<\/sub> receptors mediate the inhibitory effects of orexin\u2010A on potassium chloride\u2010induced increases in intracellular calcium ion levels in neurons derived from rat dorsal root ganglion in a chronic pain model"],"prefix":"10.1002","volume":"40","author":[{"given":"Masami","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Nihon University School of Dentistry at Matsudo Matsudo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Nihon University School of Dentistry at Matsudo Matsudo 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