{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:44:46Z","timestamp":1747151086235,"version":"3.40.5"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T00:00:00Z","timestamp":1603238400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T00:00:00Z","timestamp":1603238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["JP19590060"],"award-info":[{"award-number":["JP19590060"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JP20019016"],"award-info":[{"award-number":["JP20019016"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009023","name":"Precursory Research for Embryonic Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009023","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropsychopharm Rep"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Fear conditioning tests are intended to elucidate a subject's ability to associate a conditioned stimulus with an aversive, unconditioned stimulus, such as footshock. Among these tests, a paradigm related to precise cortical functions would be increasingly important in drug screening for disorders such as schizophrenia and dementia. Therefore, we established a new fear conditioning paradigm using a visual cue in mice. In addition, the validity of the test was evaluated using a genetically engineered mouse, heterozygous deficient in <jats:italic>Mdga1<\/jats:italic> (<jats:italic>Mdga1<\/jats:italic>+\/\u2010), which is related to schizophrenia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice were given footshocks associated with a visual cue of moving gratings at training in 25\u2010minute sessions. The mice showed the conditioned response of freezing behavior to the visual stimulus at testing 24\u00a0hours after the footshocks. In the test for validation, the <jats:italic>Mdga1<\/jats:italic>+\/\u2010\u00a0deficient mice showed significantly less freezing than wild\u2010type mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The visually cued fear conditioning paradigm with moving gratings has been established, which is experimentally useful to evaluate animal cortical functions. The validity of the test was confirmed for <jats:italic>Mdga1<\/jats:italic>\u2010deficient mice with possible deficiency in cortical functions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/npr2.12146","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T06:26:05Z","timestamp":1603347965000},"page":"371-375","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Visually cued fear conditioning test for memory impairment related to cortical function"],"prefix":"10.1002","volume":"40","author":[{"given":"Kazuya","family":"Kuboyama","sequence":"first","affiliation":[{"name":"Department of Neuropharmacology Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Sanuki city Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Shirakawa","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Sanuki city Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Kawada","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Sanuki city Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Sanuki city Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiki","family":"Ojima","sequence":"additional","affiliation":[{"name":"Department of Molecular Neurobiology Faculty of Medicine Kagawa University Kita\u2010gun Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Department of Biophysics Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Sanuki city Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Neurobiology Faculty of Medicine Kagawa University Kita\u2010gun Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1956-6069","authenticated-orcid":false,"given":"Maki K.","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Sanuki city Kagawa 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