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In this study, we examined whether a comparative study of protein expression levels can be conducted using postmortem brain samples preserved in different facilities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared the demographic factors of postmortem brain samples preserved in two institutions and measured and compared the expression levels of glyceraldehyde\u20103\u2010phosphate dehydrogenase (<jats:styled-content style=\"fixed-case\">GAPDH<\/jats:styled-content>) and glial fibrillary acidic protein (<jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content>) in the prefrontal cortex and superior temporal gyrus. <jats:styled-content style=\"fixed-case\">GAPDH<\/jats:styled-content> is generally used as a loading control for western blotting, and <jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content> is considered as an astrocyte marker in the brain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found significant differences between the two institutions in postmortem interval, age at death, and preservation time. To reduce the effects of these differences on our measurements, the parameters were set as covariates in our analyses of covariance. Subsequently, no differences in <jats:styled-content style=\"fixed-case\">GAPDH<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content> expression were found between institutions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>When studies are conducted using brain samples preserved in different brain banks, differences in demographic factors should be carefully considered and taken into account by statistical methods to minimize their impact as much as possible. Since there was no significant difference in the protein expression levels of <jats:styled-content style=\"fixed-case\">GAPDH<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content> in either region between the two institutions that preserved the postmortem brains, we concluded that it is possible to perform protein quantitative analysis assuming that there is no effect of difference between two institutions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/npr2.12430","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T11:03:56Z","timestamp":1711969436000},"page":"399-409","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Availability of individual proteins for quantitative analysis in postmortem brains preserved in two different brain 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