{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T01:58:14Z","timestamp":1788400694969,"version":"build-2803163510"},"reference-count":83,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T00:00:00Z","timestamp":1763856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004697","name":"Hamadan University of Medical Sciences","doi-asserted-by":"publisher","award":["140206144762"],"award-info":[{"award-number":["140206144762"]}],"id":[{"id":"10.13039\/501100004697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropsychopharm Rep"],"published-print":{"date-parts":[[2025,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n \n Multiple sclerosis (\n MS<\/jats:styled-content>\n ) is characterized by chronic neuroinflammation and oxidative stress. Vitamin D is believed to exert immunomodulatory and antioxidant effects, yet its impact on specific inflammatory proteins such as\n CHI3L1<\/jats:styled-content>\n (\n YKL<\/jats:styled-content>\n \u201040) in\n MS<\/jats:styled-content>\n remains unclear. This study evaluated whether 8\u2010week vitamin D3 supplementation affects serum\n CHI3L1<\/jats:styled-content>\n levels, oxidative stress markers, and antioxidant enzyme activities in patients with\n MS<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this single\u2010arm pre\u2010post clinical trial, 35 patients with\n MS<\/jats:styled-content>\n (aged 30\u201356\u2009years) received oral vitamin\n D3<\/jats:styled-content>\n supplementation (50\u2009000\u2009\n IU<\/jats:styled-content>\n \/week) for 8\u2009weeks. Serum 25(\n OH<\/jats:styled-content>\n )D and\n CHI3L1<\/jats:styled-content>\n levels were determined using commercial enzyme\u2010linked immunosorbent assay (\n ELISA<\/jats:styled-content>\n ) kits. oxidative stress markers were measured pre\u2010 and post\u2010intervention using commercial colorimetric kits. Statistical analysis was performed using paired\n t<\/jats:italic>\n \u2010tests or Wilcoxon signed\u2010rank tests.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Vitamin\n D3<\/jats:styled-content>\n supplementation significantly increased serum 25(\n OH<\/jats:styled-content>\n )D levels (20.80\u2009\u00b1\u20098.6 to 39.11\u2009\u00b1\u200912.26\u2009ng\/\n mL<\/jats:styled-content>\n ;\n p<\/jats:italic>\n \u2009<\u20090.001).\n CHI3L1<\/jats:styled-content>\n concentration decreased by 21.7% (33.28\u2009\u00b1\u20098.9 to 26.05\u2009\u00b1\u20099.1\u2009ng\/\n mL<\/jats:styled-content>\n ;\n p<\/jats:italic>\n \u2009<\u20090.001). oxidative stress was reduced, evidenced by lower\n TOS<\/jats:styled-content>\n (1.55\u2009\u00b1\u20090.50 to 0.59\u2009\u00b1\u20090.23\u2009mmol H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n equiv.\/L;\n p<\/jats:italic>\n \u2009<\u20090.001) and\n MDA<\/jats:styled-content>\n (0.08\u2009\u00b1\u20090.03 to 0.05\u2009\u00b1\u20090.026\u2009nmol\/\n mL<\/jats:styled-content>\n ;\n p<\/jats:italic>\n \u2009<\u20090.001). Antioxidant capacity improved, as demonstrated by elevated\n TAC<\/jats:styled-content>\n (0.622\u2009\u00b1\u20090.138 to 0.797\u2009\u00b1\u20090.15\u2009mmol Fe\n 2+<\/jats:sup>\n \/L;\n p<\/jats:italic>\n \u2009<\u20090.001) and increased activities of\n SOD<\/jats:styled-content>\n (10.5%;\n p<\/jats:italic>\n \u2009<\u20090.001),\n CAT<\/jats:styled-content>\n (19.5%;\n p<\/jats:italic>\n \u2009<\u20090.001), and\n GPx<\/jats:styled-content>\n (35.6%;\n p<\/jats:italic>\n \u2009<\u20090.05). Significant inverse correlations were observed between serum 25(\n OH<\/jats:styled-content>\n )D and\n CHI3L1<\/jats:styled-content>\n (\n r<\/jats:italic>\n \u2009=\u2009\u22120.999,\n p<\/jats:italic>\n \u2009<\u20090.001),\n TOS<\/jats:styled-content>\n (\n r<\/jats:italic>\n \u2009=\u2009\u22120.456,\n p<\/jats:italic>\n \u2009=\u20090.0058), and\n MDA<\/jats:styled-content>\n (\n r<\/jats:italic>\n \u2009=\u2009\u22120.577,\n p<\/jats:italic>\n \u2009<\u20090.001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Vitamin\n D3<\/jats:styled-content>\n supplementation was associated with reductions in\n CHI3L1<\/jats:styled-content>\n and oxidative stress markers, while suggesting enhancement of antioxidant capacity. This observed biomarker changes support vitamin\n D3<\/jats:styled-content>\n as a potential adjunct therapy targeting interconnected pathological pathways in\n MS.<\/jats:styled-content>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/npr2.70076","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T07:37:30Z","timestamp":1763969850000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Vitamin\n D3<\/scp>\n Supplementation Modulates Inflammatory Protein\n CHI3L1<\/scp>\n \/\n YKL<\/scp>\n \u201040 and Oxidative Stress Status in Multiple Sclerosis"],"prefix":"10.1002","volume":"45","author":[{"given":"Sevda","family":"Asadpour","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Medicine School Hamadan University of Medical Sciences Hamadan 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Center, Institute of Health Sciences and Technology, Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2183-5122","authenticated-orcid":false,"given":"Gholamreza","family":"Shafiee","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Medicine School, Nutrition Health Research Center, Hamadan University of Medical Sciences Hamadan 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