{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T11:24:23Z","timestamp":1778671463293,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T00:00:00Z","timestamp":1148947200000},"content-version":"vor","delay-in-days":5203,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Natural Toxins"],"published-print":{"date-parts":[[1992,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The toxin Ts II from the venom of the Brazilian scorpion <jats:italic>Tityus serrulatus<\/jats:italic> was purified in two successive chromatographic steps. The amino acid sequence was then determined by automated Edman degradation of the reduced and S\u2010carboxymethylated protein and of proteolytic peptides derived from it. This sequence appears to differ from that of previously characterized toxins found in this venom. However, it is identical to the recently published sequence of protein III\u20108 from the same venom [Possani et al., J Biol Chem 266:3178\u20133185, 1991], except that the C\u2010terminus was found to be amidated. Homologies were found between the sequence of Ts II and that of other toxins from <jats:italic>Tityus<\/jats:italic>; in particular, the amino acid sequence of Ts II displays 72% sequence identity with Ts VII (also c\u00e4lled TiTx \u03b3). Consistent with this structural similarity, some biological properties of Ts II were found to be similar to those of Ts VII: Ts II has an intracerebroventricular LD<jats:sub>50<\/jats:sub> of 6 ng, as compared to 0.6 ng for Ts VII; in a receptor binding assay Ts II, like Ts VII, was found to behave as a \u03b2\u2010type toxin and to inhibit the binding of the reference labelled toxin with a K<jats:sub>0.5<\/jats:sub> of 5 \u00d7 10<jats:sup>\u22129<\/jats:sup> M, as compared to 7 \u00d7 10<jats:sup>\u221211<\/jats:sup> M for Ts VII. Nevertheless, Ts II is unable to bind to anti\u2010Ts VII antibodies in radioimmunoassay experiments, indicating the non\u2010conservation between the two toxins of at least some antigenically important residues. In fact, the sequence differences between Ts II and Ts VII are mainly mapped in two regions believed to be antigenic by analogy with other scorpion toxins. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/nt.2620010211","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T02:11:27Z","timestamp":1161137487000},"page":"119-125","source":"Crossref","is-referenced-by-count":26,"title":["The \u03b2\u2010type toxin Ts II from the scorpion <i>Tityus serrulatus<\/i>: Amino acid sequence determination and assessment of biological and antigenic properties"],"prefix":"10.1002","volume":"1","author":[{"given":"Pascal","family":"Mansuelle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010France","family":"Martin\u2010Eauclaire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Chavez\u2010Olortegui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Elena","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Rochat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Granier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(86)90133-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"12553","DOI":"10.1016\/S0021-9258(18)33546-4","article-title":"Tityus serrulatus venom contains two classes of toxins\u2014Tityus \u03b3 is a new tool with a very high affinity for studying the Na+ channel","volume":"257","author":"Barhanin J","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91211-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(76)90355-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1983.tb02084.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(86)80112-7"},{"key":"e_1_2_1_8_1","first-page":"379","volume-title":"Arthropod Venoms","author":"Diniz CR","year":"1978"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(84)90077-4"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb09488.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(80)80082-9"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb14735.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/nt.2620010112"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01025013"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"4452","DOI":"10.1016\/S0021-9258(18)61214-1","article-title":"Purification and chemical and biological characterization of seven toxins from the mexican scorpion, Centruroides suffusus suffusus","volume":"262","author":"Martin M\u2010F","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1970.tb01111.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02906497"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2290739"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1016\/S0021-9258(18)49971-1","article-title":"Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom","volume":"266","author":"Possani L","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(77)90192-0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(83)90011-9"},{"key":"e_1_2_1_22_1","first-page":"100","article-title":"Structure\u2010function relationships in scorpion neurotoxins. Identification of a superreactive lysine residue in toxin I of Androctonus australis Hector","volume":"335","author":"Sampieri F","year":"1978","journal-title":"Biochem Biophys Acta"}],"container-title":["Natural Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnt.2620010211","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nt.2620010211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T03:26:22Z","timestamp":1698117982000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nt.2620010211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,3]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,3]]}},"alternative-id":["10.1002\/nt.2620010211"],"URL":"https:\/\/doi.org\/10.1002\/nt.2620010211","archive":["Portico"],"relation":{},"ISSN":["1056-9014","1522-7189"],"issn-type":[{"value":"1056-9014","type":"print"},{"value":"1522-7189","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,3]]}}}