{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:36:30Z","timestamp":1790498190672,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T00:00:00Z","timestamp":1148947200000},"content-version":"vor","delay-in-days":4716,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Natural Toxins"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>With the aim of developing active protection against the noxious effects provoked in humans by scorpion stings, the possibility of eliciting toxin reactive antibodies by immunization with a short peptide was assessed in mice. The amino acid sequence of residues 50 to 59 of <jats:italic>Androctonus australis Hector<\/jats:italic> toxin II was chosen, on the basis of previous results indicating that rabbit anti\u2010(50\u201359) antibodies neutralize the biological effects of the parent toxin. The peptide was prepared by solid\u2010phase synthesis procedures and used in different forms (free, linearly polymerized, coupled to KLH, coupled to a low\u2010molecular weight B\u2010lymphocyte activator) in order to immunize groups of non\u2010congenic NMRI or congenic C57BL\/6 mice. The reactivities of each serum with the peptide and with the toxin were assessed in ELISA. Strong reactivities with both the peptide (mean titer over 1:52,600) and the toxin (mean titer 1:800) were observed in all mice from the group that received the KLH\u2010coupled peptide. However, mouse immune sera failed either to recognize the toxin in a liquid\u2010phase radioimmunoassay or to neutralize the lethal effects of the toxin. The requirements, in terms of affinity and recognition of native conformation, for anti\u2010peptide antibodies to display neutralizing properties are discussed. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/nt.2620010410","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T02:10:05Z","timestamp":1161137405000},"page":"255-262","source":"Crossref","is-referenced-by-count":9,"title":["Antibodies cross\u2010reactive with the scorpion\u2010toxin ii from <i>Androctonus australis Hector<\/i> elicited in mice by a synthetic peptide"],"prefix":"10.1002","volume":"1","author":[{"given":"Djamel","family":"Ait\u2010Amara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Chavez\u2010Olortegui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9gine","family":"Romi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Mery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Brugidou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Albericio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Devaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Granier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,5,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1989.tb01500.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.4049\/jimmunol.136.9.3371","article-title":"Specificity and neutralizing capacity of antibodies elicited by a synthetic peptide of scorpion toxin","volume":"136","author":"Bahraoui E","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.4049\/jimmunol.141.1.214","article-title":"Monoclonal antibodies to scorpion toxins. 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