{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T06:11:43Z","timestamp":1775628703494,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T00:00:00Z","timestamp":1148947200000},"content-version":"vor","delay-in-days":4167,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Natural Toxins"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A basic myotoxic protein was purified from <jats:italic>Bothrops asper venom<\/jats:italic>. Like other basic <jats:italic>Bothrops<\/jats:italic> myotoxins, myotoxin IV induces acute muscle damage after intramuscular injection in mice and disrupts negatively charged liposomes but not positively charged ones. Furthermore, this protein exerts a weak anticoagulant effect only at concentrations of 40 \u03bcg\/ml or higher, and is devoid of phospholipase A<jats:sub>2<\/jats:sub> activity. Rabbit polyclonal antibodies to <jats:italic>B. asper<\/jats:italic> myotoxin II, a related lysine\u201049 isoform, cross\u2010react strongly with myotoxin IV by enzyme immunoassay, indicating a high degree of antigenic similarity between these two proteins. The fact that both toxins have similar amino acid compositions and amino\u2010terminal sequences, including leucine\u20105 and glutamine\u201011, 2 amino acid residues conserved in all lysine\u201049 phospholipase A<jats:sub>2<\/jats:sub> variants purified, strongly suggests that <jats:italic>B. asper<\/jats:italic> myotoxin IV is a lysine\u201049 phospholipase A<jats:sub>2<\/jats:sub>. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/nt.2620030107","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T02:33:34Z","timestamp":1161138814000},"page":"26-31","source":"Crossref","is-referenced-by-count":34,"title":["Purification and characterization of myotoxin IV, a phospholipase A<sub>2<\/sub> variant, from <i>Bothrops asper<\/i> snake venom"],"prefix":"10.1002","volume":"3","author":[{"given":"Cecilia","family":"Diaz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Lomonte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Zamudio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,30]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"211","article-title":"Degenerative and regenerative changes in murine skeletal muscle after injection of venom from the snake Bothrops asper: A histochemical and immunocytochemical study","volume":"72","author":"Arce V","year":"1991","journal-title":"Int J Exp Pathol"},{"key":"e_1_2_1_3_1","first-page":"165","article-title":"Las serpientes venenosas de Centroam\u00e9rica y el problema del ofidismo. 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