{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T06:20:53Z","timestamp":1788589253666,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,20]],"date-time":"2013-09-20T00:00:00Z","timestamp":1379635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Victorian Government's Operational Infrastructure Support Program"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>PGC\u20101\u03b1 is a transcriptional co\u2010activator and master regulator of mitochondrial biogenesis. While extensively studied in skeletal and cardiac muscle, recent findings suggest that white adipose tissue PGC\u20101\u03b1 plays an important role in regulating glucose homeostasis. The purpose of the present investigation was to evaluate the role of AMPK in regulating PGC\u20101\u03b1 and mitochondrial enzymes in mouse epididymal and inguinal subcutaneous adipose tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mitochondrial protein content and norepinephrine and CL 316,243\u2010induced PGC\u20101\u03b1 mRNA expression were studied in mouse epididymal and inguinal adipose tissue from wild\u2010type and AMPK \u03b21<jats:sup>\u2212\/\u2212<\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The protein content and phosphorylation of AMPK\u03b1 was reduced in epididymal adipose tissue from AMPK \u03b21<jats:sup>\u2212\/\u2212<\/jats:sup> compared to WT mice, concomitant with decreases in PGC\u20101\u03b1 and mitochondrial marker proteins. Norepinephrine and CL 316,243\u2010mediated induction of PGC\u20101\u03b1 were decreased in cultured epididymal adipose tissue from AMPK \u03b21<jats:sup>\u2212\/\u2212<\/jats:sup> relative to WT mice. In inguinal adipose tissue from AMPK \u03b21<jats:sup>\u2212\/\u2212<\/jats:sup> mice, mitochondrial marker protein content and norepinephrine and CL 316,243\u2010mediated increases in PGC\u20101\u03b1 were normal despite reductions in the content and phosphorylation of AMPK\u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Norepinephrine\u2010 and CL 316,243\u2010mediated induction of PGC\u20101\u03b1 and mitochondrial protein expression is regulated by AMPK in epididymal, but not inguinal adipose tissue.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.20605","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T16:32:53Z","timestamp":1377016373000},"page":"730-738","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":135,"title":["Evidence for the role of AMPK in regulating PGC\u20101 alpha expression and mitochondrial proteins in mouse epididymal adipose tissue"],"prefix":"10.1002","volume":"22","author":[{"given":"Zhongxiao","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Human Health and Nutritional Sciences University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared","family":"Root\u2010Mccaig","sequence":"additional","affiliation":[{"name":"Department of Human Health and Nutritional Sciences University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Castellani","sequence":"additional","affiliation":[{"name":"Department of Human Health and Nutritional Sciences University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce E.","family":"Kemp","sequence":"additional","affiliation":[{"name":"St Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne Fitzroy Victoria 3065 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