{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:42:43Z","timestamp":1791114163991,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T00:00:00Z","timestamp":1386547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The study aims to explore long\u2010term dietary effects on increases in body mass and fat depot enlargement through the recruitment of early in life labeled progenitor cells to the adipolineage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Neonate male C57BL\/6J (B6) mice were injected intraperitoneally with BrdU. From 4 until 30 weeks of age they were fed either low fat diet (LFD) or high fat diet (HFD). BrdU\u2010labeled cells were analyzed by flow cytometric and immunohistochemical assays after 10 days and 4, 8, 16, and 30 weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice fed HFD were heavier than mice fed LFD with the most dramatic disparity recorded between week 16 and 30. BrdU\u2010bearing cells showed the decrease in the percentage content of labeled cells in inguinal (iWAT), epididymal (eWAT) and bone marrow (BM) tissues, regardless diets. However, iWAT collected from animals on HFD showed significant increase in labeled\u2010cells at week 16th, which coincides with robust increase in inguinal but not epididymal fat weight between 16 and 30 weeks age.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cells labeled with BrdU during neonate life of B6 mice persist in fat tissues for long period of time and are recruited to the adipocyte lineage in a favorable (obesogenic) environment in iWAT but not in eWAT.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.20671","type":"journal-article","created":{"date-parts":[[2013,11,26]],"date-time":"2013-11-26T03:38:33Z","timestamp":1385437113000},"page":"1091-1102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Recruitment of fat cell precursors during high fat diet in C57BL\/6J mice is fat depot specific"],"prefix":"10.1002","volume":"22","author":[{"given":"B.","family":"Gawronska\u2010Kozak","sequence":"first","affiliation":[{"name":"Department of Biological Function of Food Institute of Animal Reproduction and Food Research of Polish Academy of Sciences Olsztyn Poland"},{"name":"Department of Stem Cell &amp; Developmental Biology Regenerative Biology Laboratory, Pennington Biomedical Research Center Louisiana State University System Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Staszkiewicz","sequence":"additional","affiliation":[{"name":"Department of Stem Cell &amp; Developmental Biology Regenerative Biology Laboratory, Pennington Biomedical Research Center Louisiana State University System Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Gimble","sequence":"additional","affiliation":[{"name":"Department of Stem Cell &amp; Developmental BiologyStem Cell Biology Laboratory, Pennington Biomedical Research Center Louisiana State University System Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Kirk\u2010Ballard","sequence":"additional","affiliation":[{"name":"Department of Stem Cell &amp; Developmental Biology Regenerative Biology Laboratory, Pennington Biomedical Research Center Louisiana State University System Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1978.235.3.E279"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/S0022-2275(20)37807-X","article-title":"Demonstration of de novo production of adipocytes in adult rats by biochemical and radioautographic techniques","volume":"25","author":"Miller WH","year":"1984","journal-title":"J Lipid Res"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608144200"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06902"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/81.3.555"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.6.8567"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.004"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.11.021"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1753"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.190"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00202.2006"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601752103"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0411"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.11.092"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00703"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0270"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2010.0507"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-1027"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/616193"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31021"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/79111"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0219"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.8.1958"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2008.04.004"}],"container-title":["Obesity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Foby.20671","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Foby.20671","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/oby.20671","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T02:40:16Z","timestamp":1696387216000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/oby.20671"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,9]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1002\/oby.20671"],"URL":"https:\/\/doi.org\/10.1002\/oby.20671","archive":["Portico"],"relation":{},"ISSN":["1930-7381","1930-739X"],"issn-type":[{"value":"1930-7381","type":"print"},{"value":"1930-739X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,9]]},"assertion":[{"value":"2013-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}