{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:10:20Z","timestamp":1785215420097,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T00:00:00Z","timestamp":1395014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Circulating branched\u2010chain amino acids (BCAAs) are elevated in obesity and this has been linked to obesity comorbidities. However it is unclear how obesity affects alloisoleucine, a BCAA and pathognomonic marker of branched\u2010chain keto acid dehydrogenase complex (BCKDC) disorders. It has been previously established that obese Zucker rats exhibit BCKDC impairments in fat and other tissues, whereas BCKDC impairments in adipose tissue of DIO rats are compensated by increased hepatic BCKDC activity. Therefore, alloisoleucine was investigated in these two obesity models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Amino acids were extracted from plasma and measured using ultra performance liquid chromatography mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Plasma alloisoleucine was 238% higher in obese compared to lean Zucker rats. This elevation was greater than that of other BCAAs (107\u2010124%). DIO rats had no significant change in alloisoleucine, despite elevations in other BCAAs (15\u201066%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Alloisoleucine was elevated in obese Zucker but not DIO rats consistent with known global impairments of BCKDC in Zucker but not DIO rats. Cytotoxic branched\u2010chain ketoacids (BCKAs) accumulate in genetic disorders affecting BCKDC. BCKAs increase reactive oxygen species, stress kinase activation, and mitochondrial dysfunction. Inasmuch as these factors underlie obesity comorbidities, it may important to identify obese individuals with elevated alloisoleucine.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.20691","type":"journal-article","created":{"date-parts":[[2014,1,11]],"date-time":"2014-01-11T07:12:40Z","timestamp":1389424360000},"page":"1212-1215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Alloisoleucine differentiates the branched\u2010chain aminoacidemia of Zucker and dietary obese rats"],"prefix":"10.1002","volume":"22","author":[{"given":"Kristine C.","family":"Olson","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Physiology Penn State University College of Medicine, Hershey  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences Penn State University College of Medicine, Hershey  Pennsylvania USA"},{"name":"The Macromolecular Core Facility Penn State University College of Medicine, Hershey  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology Penn State University College of Medicine, Hershey  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andras","family":"Hajnal","sequence":"additional","affiliation":[{"name":"Department of Neural and Behavioral Sciences Penn State University College of Medicine, Hershey  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology Penn State University College of Medicine, Hershey  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.1.319S"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1668-09.2009"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.02.002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2307"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.111.000737"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orcp.2013.07.003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00630.2012"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059443"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00134.2007"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2013.05.010"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.05.167"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22242"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-005-4152-8"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.06.006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl381"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.000586"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00246-010-9856-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.10.1734"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2007.098434"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"22141","DOI":"10.1016\/S0021-9258(18)41646-8","article-title":"On the mechanisms of the formation of L\u2010alloisoleucine and the 2\u2010hydroxy\u20103\u2010methylvaleric acid stereoisomers from l\u2010isoleucine in maple syrup urine disease patients and in normal humans","volume":"267","author":"Mamer OA","year":"1992","journal-title":"J Biol Chem"}],"container-title":["Obesity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Foby.20691","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/oby.20691","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T05:50:29Z","timestamp":1696485029000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/oby.20691"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,17]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1002\/oby.20691"],"URL":"https:\/\/doi.org\/10.1002\/oby.20691","archive":["Portico"],"relation":{},"ISSN":["1930-7381","1930-739X"],"issn-type":[{"value":"1930-7381","type":"print"},{"value":"1930-739X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,17]]},"assertion":[{"value":"2013-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}