{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T14:49:49Z","timestamp":1769266189913,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,5,9]],"date-time":"2015-05-09T00:00:00Z","timestamp":1431129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"United States Army Medical Research and Materiel Command (MRMC)\/Telemedicine and Advanced Technology Research Center (TATRC)"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine relationships between weight loss through changes in lifestyle and peripheral blood gene expression profiles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective nonrandomized trial was conducted over 1 year in participants undergoing intensive lifestyle modification to reverse or stabilize progression of coronary artery disease. Cardiovascular risk factors, inflammatory biomarkers, and gene expression as a function of weight loss were assessed in 89 lifestyle participants and 71 retrospectively matched controls undergoing usual care.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Substantial weight loss (\u221215.2\u2009\u00b1\u20093.8%) in lifestyle participants (<jats:italic>n<\/jats:italic>\u2009=\u200933) was associated with improvement in selected cardiovascular risk factors and significant changes in peripheral blood gene expression from pre\u2010 to post\u2010intervention: 132 unique genes showed significant expression changes (false discovery rate corrected <jats:italic>P<\/jats:italic>\u2010value &lt;0.05 and fold\u2010change \u22651.4). Altered molecular pathways were related to immune function and inflammatory responses involving endothelial activation. In contrast, participants losing minimal weight (\u22123.1\u2009\u00b1\u20092.5%, <jats:italic>n<\/jats:italic>\u2009=\u200932) showed only minor changes in cardiovascular risk factors and markers of inflammation and no changes in gene expression compared to non intervention controls after 1 year.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Weight loss (\u226510%) during lifestyle modification is associated with down\u2010regulation of genetic pathways governing interactions between circulating immune cells and the vascular endothelium and may be required to successfully reduce CVD risk.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.21079","type":"journal-article","created":{"date-parts":[[2015,5,9]],"date-time":"2015-05-09T11:03:51Z","timestamp":1431169431000},"page":"1312-1319","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Importance of substantial weight loss for altering gene expression during cardiovascular lifestyle modification"],"prefix":"10.1002","volume":"23","author":[{"given":"Darrell L.","family":"Ellsworth","sequence":"first","affiliation":[{"name":"Integrative Cardiac Health Program Windber Research Institute Windber Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly A.","family":"Mamula","sequence":"additional","affiliation":[{"name":"Integrative Cardiac Health Program Windber Research Institute Windber Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather L.","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Integrative Cardiac Health Program Windber Research Institute Windber Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fionnuala A.","family":"McDyer","sequence":"additional","affiliation":[{"name":"Almac Diagnostics, Almac Group Limited  Craigavon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gera L.","family":"Jellema","sequence":"additional","affiliation":[{"name":"Almac Diagnostics, Almac Group Limited  Craigavon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"van Laar","sequence":"additional","affiliation":[{"name":"Bioinformatics Department ChipDX LLC New York New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas S.","family":"Costantino","sequence":"additional","affiliation":[{"name":"Integrative Cardiac Health Program Windber Research Institute Windber Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata J.M.","family":"Engler","sequence":"additional","affiliation":[{"name":"Integrative Cardiac Health Program Walter Reed National Military Medical Center Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina 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