{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T09:46:55Z","timestamp":1768038415170,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,7,6]],"date-time":"2015-07-06T00:00:00Z","timestamp":1436140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre Research Incentive Fund (FIPE\/HCPA)"},{"name":"The Brazilian Federal Agency"},{"name":"Evaluation of Graduate Education (CAPES) for the graduate fellowship awarded to Sane Vianna Pereira"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to correlate different degrees of excess weight with the expression of podocyte\u2010associated messenger RNAs (mRNAs) in urine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The sample comprised 83 patients with overweight or obesity class I, II, or III and 18 healthy controls. The expression of nephrin, podocin, podocalyxin, \u03b1\u2010actinin\u20104, \u03b13\u03b21integrin, vascular endothelial growth factor, and transforming growth factor\u2010beta (TGF\u2010\u03b2<jats:sub>1<\/jats:sub>) mRNA in urine was quantified with the real\u2010time polymerase chain reaction. mRNA expression was correlated with body mass index, the metabolic syndrome, albuminuria, and inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Adults with obesity class III had higher levels of serum lipids, glucose, HbA1C, insulin resistance, and C\u2010reactive protein (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), with 85% of the subjects meeting criteria for the metabolic syndrome (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001 vs. other groups). Urinary podocyte\u2010associated mRNAs were higher in adults with obesity class III than in other groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Patients with overweight or obesity class I or II also had higher levels of podocyte mRNAs than controls: nephrin (<jats:italic>P<\/jats:italic>\u2009=\u20090.021), \u03b1\u2010actinin\u20104 (<jats:italic>P<\/jats:italic>\u2009=\u20090.014), \u03b13\u03b21integrin (<jats:italic>P<\/jats:italic>\u2009=\u20090.036), and TGF\u2010\u03b2<jats:sub>1<\/jats:sub> (<jats:italic>P<\/jats:italic>\u2009=\u20090.005). Metabolic syndrome, hyperinsulinemia, and C\u2010reactive protein were correlated with podocyturia, but only higher insulin levels were related regardless of obesity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Severe obesity and hyperinsulinemia were associated with higher urinary expression of podocyte\u2010associated mRNAs, even at normal urinary albumin excretion rates.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.21156","type":"journal-article","created":{"date-parts":[[2015,7,6]],"date-time":"2015-07-06T11:18:20Z","timestamp":1436181500000},"page":"1643-1649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Increased urine podocyte\u2010associated messenger RNAs in severe obesity are evidence of podocyte injury"],"prefix":"10.1002","volume":"23","author":[{"given":"Sane Vianna","family":"Pereira","sequence":"first","affiliation":[{"name":"Graduate Program in Medicine: Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariane","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Graduate Program in Medicine: Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Garcia","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Graduate Program in Medicine: Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan Fraportti","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Graduate Program in Medicine: Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Rodolfo Tel\u00f3","family":"Timm","sequence":"additional","affiliation":[{"name":"Graduate Program in Medicine: Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Zancan","sequence":"additional","affiliation":[{"name":"Division of Nephrology Hospital de Cl\u00ednicas de Porto Alegre Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rog\u00e9rio","family":"Friedman","sequence":"additional","affiliation":[{"name":"Division of Endocrinology Hospital de Cl\u00ednicas de Porto Alegre Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Ver\u00edssimo","family":"Veronese","sequence":"additional","affiliation":[{"name":"Graduate Program in Medicine: Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil"},{"name":"Division of Nephrology Hospital de Cl\u00ednicas de Porto Alegre Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/01913123.2011.584657"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfi201"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2012.12.006"},{"key":"e_1_2_7_5_1","first-page":"39","article-title":"Manifestation of renal disease in obesity: pathophysiology of obesity\u2010related dysfunction of the kidney","volume":"2","author":"D'Elia JA","year":"2009","journal-title":"Int J Nephrol Renovasc Dis"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000093632"},{"key":"e_1_2_7_7_1","first-page":"1645","article-title":"Adiponectin regulates albuminuria and podocyte function in mice","volume":"118","author":"Sharma K","year":"2008","journal-title":"J Clin Invest"},{"key":"e_1_2_7_8_1","first-page":"1","article-title":"Preventing and managing the global epidemic: report of a WHO consultation","volume":"894","author":"7","year":"2000","journal-title":"World Health Organ Tech Rep Ser"},{"key":"e_1_2_7_9_1","unstructured":"8. 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