{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:29:57Z","timestamp":1790681397452,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"American Diabetes Association to Dr. Ruchi Mathur","award":["1-12-IN-28"],"award-info":[{"award-number":["1-12-IN-28"]}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"crossref","award":["UL1RR033176"],"award-info":[{"award-number":["UL1RR033176"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"crossref","award":["UL1TR000124"],"award-info":[{"award-number":["UL1TR000124"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Methanogens colonizing the human gut produce methane and influence host metabolism. This study examined metabolic parameters in methane\u2010producing subjects before and after antibiotic treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eleven prediabetic methane\u2010positive subjects (9F, 2M) with obesity (BMI 35.17\u2009\u00b1\u20097.71 kg\/m<jats:sup>2<\/jats:sup>) aged 47\u2009\u00b1\u20099 years were recruited. Subjects underwent breath testing, symptom questionnaire, oral glucose tolerance test (OGTT), lipid profile, and stool <jats:italic>Methanobrevibacter smithii<\/jats:italic> levels, gastric transit, and energy utilization analyses. After a 10\u2010day antibiotic therapy (neomycin 500 mg bid\/rifaximin 550 mg tid), all testing was repeated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Baseline stool <jats:italic>M. smithii<\/jats:italic> levels correlated with breath methane (<jats:italic>R<\/jats:italic>\u2009=\u20090.7, <jats:italic>P<\/jats:italic>\u2009=\u20090.05). Eight subjects (73%) eradicated breath methane and showed reduced stool <jats:italic>M. smithii<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009=\u20090.16). After therapy, methane\u2010eradicated subjects showed significant improvements in low\u2010density lipoprotein (LDL) (<jats:italic>P<\/jats:italic>\u2009=\u20090.028), total cholesterol (P\u2009=\u20090.01), and insulin levels on OGTT (<jats:italic>P<\/jats:italic>\u2009=\u20090.05 at 120 minutes), lower blood glucose levels on OGTT (<jats:italic>P<\/jats:italic>\u2009=\u20090.054 at 90 minutes), significant reductions in bloating (<jats:italic>P<\/jats:italic>\u2009=\u20090.018) and straining (<jats:italic>P<\/jats:italic>\u2009=\u20090.059), and a trend toward lower stool dry weight. No changes were detected in gastric emptying time or energy harvest.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Breath methane eradication and <jats:italic>M. smithii<\/jats:italic> reduction are associated with significant improvements in total cholesterol, LDL, and insulin levels and with lower glucose levels in prediabetic subjects with obesity. The underlying mechanisms require further elucidation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.21385","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T11:29:19Z","timestamp":1454326159000},"page":"576-582","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Metabolic effects of eradicating breath methane using antibiotics in prediabetic subjects with obesity"],"prefix":"10.1002","volume":"24","author":[{"given":"Ruchi","family":"Mathur","sequence":"first","affiliation":[{"name":"Division of Endocrinology Cedars\u2010Sinai Medical Center Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen S.","family":"Chua","sequence":"additional","affiliation":[{"name":"GI Motility Program Cedars\u2010Sinai Medical Center Los Angeles CA 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