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At 6 weeks of age, mice were subjected to intramyocardial injection with lentivirus to down\u2010regulate the expression of either cardiac CPT\u20101b or green fluorescent protein. Morphological, biochemical, functional, histological, and ultrastructural profiles were assessed at 16 weeks of age.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HFD administration elicited obesity, cardiac hypertrophy, and systolic dysfunction accompanied with altered biochemical parameters. In addition, HFD consumption promoted lipid accumulation and reactive oxygen species generation in cardiomyocytes and damaged myocardial ultrastructure. Cardiac CPT\u20101b silencing protected against HFD\u2010induced cardiac remodeling by decreasing heart weight\/tibial length ratio and increasing left ventricular ejection fraction and fractional shortening, as well as normalizing left ventricular diameter. Meanwhile, CPT\u20101b inhibition mitigated the changes in biochemical parameters, aggravated myocardial lipid accumulation, reduced intramyocardial reactive oxygen species production, and partly amended myocardial ultrastructural alterations in obese mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cardiac CPT\u20101b suppression protects against the aggravation of cardiac morphology and function associated with HFD feeding. CPT\u20101b represents a potential therapeutic target for the treatment of cardiac dysfunction related to metabolic diseases such as obesity and diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.21665","type":"journal-article","created":{"date-parts":[[2016,11,2]],"date-time":"2016-11-02T09:28:09Z","timestamp":1478078889000},"page":"2533-2543","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Cardiac\u2010specific down\u2010regulation of carnitine palmitoyltransferase\u20101b (CPT\u20101b) prevents cardiac remodeling in obese mice"],"prefix":"10.1002","volume":"24","author":[{"given":"Yijie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiology Renmin Hospital of Wuhan University Wuhan People's Republic of China"},{"name":"Cardiovascular Research Institute, Wuhan University Wuhan People's Republic of China"},{"name":"Hubei Key Laboratory of Cardiology Wuhan People's Republic of 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