{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T21:45:54Z","timestamp":1775252754293,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,6,14]],"date-time":"2018-06-14T00:00:00Z","timestamp":1528934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"European Union's Horizon 2020 research and innovation program","award":["634880"],"award-info":[{"award-number":["634880"]}]},{"name":"Swedish Research Council, the Frimurare Barnhuset Foundation"},{"name":"Kronprinsessan Lovisas Foundation"},{"name":"European Society for Pediatric Endocrinology Research"},{"name":"Integrated Research and Treatment Center (IFB)","award":["SFB1052\/2 (B04 to NK)"],"award-info":[{"award-number":["SFB1052\/2 (B04 to NK)"]}]},{"name":"IFB Adiposity Diseases","award":["FKZ 01E01501 to NK"],"award-info":[{"award-number":["FKZ 01E01501 to NK"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin\u2010layer chromatography.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Substantial amounts of testosterone in adipose tissue (25\u2010153 ng\/g tissue) and in the supernatant of adipocytes (0.33\u20100.69 ng\/ten thousand cells]) were found. <jats:italic>StAR<\/jats:italic> and steroidogenic enzymes encoded by genes including <jats:italic>Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3<\/jats:italic>, and <jats:italic>Srd5a2<\/jats:italic> were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.22198","type":"journal-article","created":{"date-parts":[[2018,6,14]],"date-time":"2018-06-14T11:59:04Z","timestamp":1528977544000},"page":"1161-1167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8764-149X","authenticated-orcid":false,"given":"Isabel Viola","family":"Wagner","sequence":"first","affiliation":[{"name":"Pediatric Endocrinology Unit, Department of Women's and Children's Health Karolinska Institute Stockholm Sweden"},{"name":"Department of Pediatrics, Medical Faculty University of Cologne Cologne Germany"},{"name":"Integrated Research and Treatment Center (IFB Adiposity Diseases) University of Leipzig Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lena","family":"Sahlin","sequence":"additional","affiliation":[{"name":"Pediatric Endocrinology Unit, Department of Women's and Children's Health Karolinska Institute Stockholm Sweden"},{"name":"Nordfertil Research Lab Stockholm Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iuliia","family":"Savchuk","sequence":"additional","affiliation":[{"name":"Pediatric Endocrinology Unit, Department of Women's and Children's Health Karolinska Institute Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora","family":"Kl\u00f6ting","sequence":"additional","affiliation":[{"name":"Integrated Research and Treatment Center (IFB Adiposity Diseases) University of Leipzig Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin","family":"Svechnikov","sequence":"additional","affiliation":[{"name":"Pediatric Endocrinology Unit, Department of Women's and Children's Health Karolinska Institute Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olle","family":"S\u00f6der","sequence":"additional","affiliation":[{"name":"Pediatric Endocrinology Unit, Department of Women's and Children's Health Karolinska Institute Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-032046"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des028"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des232"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2007"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2012-1003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-1860"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2003-0090"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.85.6.2304"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.12524"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.12.105"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2011-1034"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2337\/db13-1514"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-3399"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2012-3908"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dms030"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmq001"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041536"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(02)02985-0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2016.03.006"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2015.03.016"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2008.03262.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-38265"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-2-1165"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2008.10.035"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00586.2009"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.2337\/db14-0744"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117841"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1413"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2008.05.057"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.5.997"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058-015-0647-3"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11695-012-0823-9"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.12.8088"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2014.12.015"},{"key":"e_1_2_6_36_1","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1210\/mend.13.6.0311","article-title":"Augmented androgen production is a stable steroidogenic phenotype of propagated theca cells from polycystic ovaries","volume":"13","author":"Nelson VL","year":"1999","journal-title":"Mol Endocrinol"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2005.06.003"}],"container-title":["Obesity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Foby.22198","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/oby.22198","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T07:58:23Z","timestamp":1695455903000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/oby.22198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,14]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1002\/oby.22198"],"URL":"https:\/\/doi.org\/10.1002\/oby.22198","archive":["Portico"],"relation":{},"ISSN":["1930-7381","1930-739X"],"issn-type":[{"value":"1930-7381","type":"print"},{"value":"1930-739X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,14]]},"assertion":[{"value":"2018-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}