{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:20:11Z","timestamp":1790968811352,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31471321"],"award-info":[{"award-number":["31471321"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009108","name":"Shandong University","doi-asserted-by":"publisher","award":["2018TB019"],"award-info":[{"award-number":["2018TB019"]}],"id":[{"id":"10.13039\/100009108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2022,11]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>Elevation of energy expenditure through an increase of brown adipose tissue (BAT) thermogenesis is regarded as one of the most promising ways to prevent obesity development. The preoptic area (POA) of the hypothalamus is a critical area for control of BAT thermogenesis. However, the intracellular signaling cascades in the POA for regulation of BAT thermogenesis are poorly understood.<\/jats:p><\/jats:sec>Methods<\/jats:title>Phosphorylation proteomics (phosphoproteomics) and bioinformatics approaches were used to disclose numerous hypothalamic signaling pathways involved in the regulation of BAT thermogenesis. Conditional manipulation of the p38\u03b1 gene in mouse POA was performed by stereotaxic injection of adeno\u2010associated virus 9 vector to explore the role of p38\u03b1 in BAT thermogenesis.<\/jats:p><\/jats:sec>Results<\/jats:title>Multiple hypothalamic signaling pathways were triggered by cold exposure, especially the mitogen\u2010activated protein kinase (MAPK) signaling pathway. The p38\u03b1 activation, but not extracellular signal\u2010regulated kinase 1\/2 (ERK1\/2) and c\u2010Jun NH2\u2010terminal kinase (JNK), in the hypothalamus was significantly decreased during cold exposure. p38\u03b1 deficiency in the POA dramatically elevated energy expenditure owing to a marked increase in BAT thermogenesis, resulting in significantly decreased body weight gain and fat mass. Overexpression of p38\u03b1 in the POA led to a dramatic increase in weight gain.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results demonstrate that p38\u03b1 in the POA exacerbates obesity development, at least in part owing to a decrease in BAT thermogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.23552","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T07:17:29Z","timestamp":1667373449000},"page":"2242-2255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["p38\u03b1 in the preoptic area inhibits brown adipose tissue<\/scp> thermogenesis"],"prefix":"10.1002","volume":"30","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Endocrinology Medical Integration and Practice Center & Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University Jinan China"},{"name":"Shandong Key Laboratory of Endocrinology 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