{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:51:03Z","timestamp":1786978263351,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T00:00:00Z","timestamp":1678060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CP19\/00098"],"award-info":[{"award-number":["CP19\/00098"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI21\/00003"],"award-info":[{"award-number":["FI21\/00003"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["INT21\/00078"],"award-info":[{"award-number":["INT21\/00078"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI20\/00505"],"award-info":[{"award-number":["PI20\/00505"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Alterations in the hepatic lipidome are a crucial factor involved in the pathophysiology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the serum and hepatic profile of branched\u2010chain fatty acids (BCFAs) in patients with different stages of NAFLD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a case\u2013control study performed in 27 patients without NAFLD, 49 patients with nonalcoholic fatty liver,\u00a0and 17 patients with nonalcoholic steatohepatitis, defined by liver biopsies. Serum and hepatic levels of BCFAs were analyzed by gas chromatography\u2013mass spectrometry. The hepatic expression of genes involved in the endogenous synthesis of BCFAs was analyzed by\u00a0real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A significant increase in hepatic BCFAs was found in subjects with NAFLD compared with those without NAFLD; no differences were observed in serum BCFAs between study groups. Trimethyl BCFAs, iso\u2010BCFAs, and anteiso\u2010BCFAs were increased in subjects with NAFLD (either nonalcoholic fatty liver or nonalcoholic steatohepatitis) compared with those without NAFLD. Correlation analysis showed a relationship between hepatic BCFAs and the histopathological diagnosis of NAFLD, as well as other histological and biochemical parameters related to this disease. Gene expression analysis in liver showed that the mRNA levels of\n                      <jats:italic>BCAT1<\/jats:italic>\n                      ,\n                      <jats:italic>BCAT2<\/jats:italic>\n                      , and\n                      <jats:italic>BCKDHA<\/jats:italic>\n                      were upregulated in patients with NAFLD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results suggest that the increased production of liver BCFAs might be related to NAFLD development and progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/oby.23711","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T05:57:30Z","timestamp":1678082250000},"page":"1064-1074","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Hepatic and serum\n                    <scp>branched\u2010chain<\/scp>\n                    fatty acid profile in patients with\n                    <scp>nonalcoholic<\/scp>\n                    fatty liver disease: A\n                    <scp>case\u2013control<\/scp>\n                    study"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9761-6888","authenticated-orcid":false,"given":"Jos\u00e9 Ignacio","family":"Mart\u00ednez\u2010Montoro","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Nutrition, Virgen de la Victoria University Hospital, Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga (IBIMA), Faculty of Medicine University of M\u00e1laga  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8938-2767","authenticated-orcid":false,"given":"Mar\u00eda \u00c1ngeles","family":"N\u00fa\u00f1ez\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Research Laboratory Biomedical Research Institute of Murcia (IMIB)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Antonia","family":"Martinez\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Research Laboratory Biomedical Research Institute of Murcia (IMIB)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Balaguer\u2010Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Research Laboratory Biomedical Research Institute of Murcia (IMIB)  Murcia Spain"},{"name":"Department of General and Digestive System Surgery Virgen de la Arrixaca University Hospital  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia E.","family":"Fern\u00e1ndez\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Research Laboratory Biomedical Research Institute of Murcia (IMIB)  Murcia Spain"},{"name":"Department of Endocrinology and Nutrition Virgen de la Arrixaca University Hospital  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Ferrer\u2010G\u00f3mez","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Research Laboratory Biomedical Research Institute of Murcia (IMIB)  Murcia Spain"},{"name":"Department of Endocrinology and Nutrition Virgen de la Arrixaca University Hospital  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Sledzinski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biochemistry, Faculty of Pharmacy Medical University of Gdansk  Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Dolores","family":"Frutos","sequence":"additional","affiliation":[{"name":"Department of General and Digestive System Surgery Virgen de la Arrixaca University Hospital  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2229-8488","authenticated-orcid":false,"given":"Jos\u00e9 Carlos","family":"Fern\u00e1ndez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Nutrition, Regional University Hospital of M\u00e1laga, Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga (IBIMA), Faculty of Medicine University of M\u00e1laga  M\u00e1laga Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de la Fisiopatolog\u00eda de la Obesidad y la Nutrici\u00f3n (CIBERObn) Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Mika","sequence":"additional","affiliation":[{"name":"Department of 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