{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:30:18Z","timestamp":1785796218311,"version":"3.56.0"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8157041114"],"award-info":[{"award-number":["8157041114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>MicroRNA 484 (miR\u2010484) plays a pivotal role in the development and progression of different diseases and is typically described as a mitochondrial regulator. Whether miR\u2010484 is involved in lipid metabolism or exerts a role in nonalcoholic fatty liver disease remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>miR\u2010484 levels were examined in the livers of male mice fed a high\u2010fat diet and in hepatocytes treated with free fatty acids. Sorbin and SH3 structural domain\u2010containing protein 2 (Sorbs2) were identified as a novel target of miR\u2010484 by sequencing mRNA in the livers of miR\u2010484 knockout mice. Sorbs2 liver\u2010specific knockdown mice were constructed by tail vein injection of adeno\u2010associated virus vector to miR\u2010484 knockout mice. In addition, genetic manipulation of SORBS2 was performed in human hepatocyte lines, mouse primary hepatocytes, and the liver.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum and hepatic miR\u2010484 levels are upregulated in nonalcoholic fatty liver disease mice. miR\u2010484 knockdown ameliorated hepatocyte steatosis, whereas miR\u2010484 overexpression increased hepatocyte lipid load. miR\u2010484 knockdown\u2010mediated alleviation of hepatic steatosis, liver injury, inflammation, and apoptosis was compromised after high\u2010fat diet\u2010induced knockdown of Sorbs2 in mouse liver and free fatty acid\u2010induced primary mouse hepatocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results identify Sorbs2\u2010mediated mitochondrial \u03b2\u2010oxidation and apoptosis that promote miR\u2010484 knockdown\u2010mediated remission of hepatic steatosis.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" 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