{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:28:42Z","timestamp":1792175322806,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170593"],"award-info":[{"award-number":["82170593"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100606"],"award-info":[{"award-number":["82100606"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["2020CXJQ01"],"award-info":[{"award-number":["2020CXJQ01"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Metabolic dysfunction\u2010associated steatotic liver disease (MASLD) is becoming an escalating health problem in pediatric populations. This study aimed to investigate the role of N\u2010acetyltransferase 10 (NAT10) in maternal high\u2010fat diet (HFD)\u2010induced MASLD in offspring at early life.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We generated male hepatocyte\u2010specific NAT10 knockout (<jats:italic>Nat10<\/jats:italic><jats:sup>HKO<\/jats:sup>) mice and mated them with female <jats:italic>Nat10<\/jats:italic><jats:sup>fl\/fl<\/jats:sup> mice under chow or HFD feeding. Body weight, liver histopathology, and expression of lipid metabolism\u2013associated genes (<jats:italic>Srebp1c<\/jats:italic>, <jats:italic>Fasn<\/jats:italic>, <jats:italic>Ppar\u03b1<\/jats:italic>, <jats:italic>Cd36<\/jats:italic>, <jats:italic>Fatp2<\/jats:italic>, <jats:italic>Mttp<\/jats:italic>, and <jats:italic>Apob<\/jats:italic>) were assessed in male offspring at weaning. Lipid uptake assays were performed both in vivo and in vitro. The mRNA stability assessment and RNA immunoprecipitation were performed to determine NAT10\u2010regulated target genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NAT10 deletion in hepatocytes of male offspring alleviated perinatal lipid accumulation induced by maternal HFD, decreasing expression levels of <jats:italic>Srebp1c<\/jats:italic>, <jats:italic>Fasn<\/jats:italic>, <jats:italic>Cd36<\/jats:italic>, <jats:italic>Fatp2<\/jats:italic>, <jats:italic>Mttp<\/jats:italic>, and <jats:italic>Apob<\/jats:italic> while enhancing <jats:italic>Ppar\u03b1<\/jats:italic> expression. Furthermore, <jats:italic>Nat10<\/jats:italic><jats:sup>HKO<\/jats:sup> male mice exhibited reduced lipid uptake. In vitro, NAT10 promoted lipid uptake by enhancing the mRNA stability of CD36 and FATP2. RNA immunoprecipitation assays exhibited direct interactions between NAT10 and CD36\/FATP2 mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>NAT10 deletion in offspring hepatocytes ameliorates maternal HFD\u2010induced hepatic steatosis through decreasing mRNA stability of CD36 and FATP2, highlighting NAT10 as a potential therapeutic target for pediatric MASLD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.24041","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T05:29:49Z","timestamp":1717133389000},"page":"1349-1361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Loss of <scp>NAT10<\/scp> alleviates maternal high\u2010fat diet\u2010induced hepatic steatosis in male offspring of mice"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7202-9625","authenticated-orcid":false,"given":"Qian\u2010Ren","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Fatty Liver, Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Fatty Liver, Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Shen","sequence":"additional","affiliation":[{"name":"Center for Fatty Liver, Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 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