{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:07:21Z","timestamp":1790816841436,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG078768"],"award-info":[{"award-number":["AG078768"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Apolipoprotein A4 (APOA4) is synthesized by the small intestine in response to dietary lipids. Chronic exposure to a high\u2010fat diet (HFD) desensitizes lipid\u2010induced APOA4 production and attenuates brown adipose tissue (BAT) thermogenesis. We hypothesized that exogenous APOA4 could increase BAT thermogenesis and energy expenditure in HFD\u2010fed mice, resulting in decreased obesity and improved glucose tolerance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BAT and inguinal white adipose tissue (IWAT) thermogenesis, body composition, energy intake and expenditure, and locomotor activity were measured using an infrared camera, immunoblots, quantitative magnetic resonance imaging, and a comprehensive lab animal monitoring system. An intraperitoneal glucose tolerance test and hepatic lipid accumulation and steatosis were assayed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice receiving continuous infusion of APOA4 for the last 4\u2009weeks of 10\u2009weeks of HFD feeding gained no additional body weight and had reduced fat mass but enhanced BAT and IWAT thermogenesis and energy expenditure, despite unaltered food intake and locomotor activity. Additionally, APOA4 infusion elevated fatty acid \u03b2 oxidation; decreased lipogenesis, lipid accumulation, and steatosis in liver; and improved glucose tolerance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Maintenance of plasma APOA4 via exogenous APOA4 protein parallels elevation of BAT and IWAT thermogenesis, hepatic fatty acid \u03b2 oxidation, and overall energy expenditure, with subsequent prevention of additional weight gain in HFD\u2010fed obese mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/oby.24155","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T04:34:44Z","timestamp":1731558884000},"page":"2321-2333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Attenuation of high\u2010fat diet\u2010induced weight gain by apolipoprotein <scp>A4<\/scp>"],"prefix":"10.1002","volume":"32","author":[{"given":"Hsuan\u2010Chih N.","family":"Kuo","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Diabetes Institute, Heritage College of Osteopathic Medicine Ohio University  Athens Ohio USA"},{"name":"Department of Biological Sciences Ohio University  Athens Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2085-3591","authenticated-orcid":false,"given":"Zachary","family":"LaRussa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Diabetes Institute, Heritage College of Osteopathic Medicine Ohio University  Athens Ohio USA"},{"name":"Department of Biological Sciences Ohio University  Athens Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flora Mengyang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biology Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2831-9999","authenticated-orcid":false,"given":"Leslie A.","family":"Consitt","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Diabetes Institute, Heritage College of Osteopathic Medicine Ohio University  Athens Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2505-0218","authenticated-orcid":false,"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Cincinnati  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2756-2989","authenticated-orcid":false,"given":"W. 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