{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T07:56:20Z","timestamp":1775116580835,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006955","name":"Office of Extramural Research","doi-asserted-by":"publisher","award":["RF1AG068629"],"award-info":[{"award-number":["RF1AG068629"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006955","name":"Office of Extramural Research","doi-asserted-by":"publisher","award":["AG061805"],"award-info":[{"award-number":["AG061805"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006955","name":"Office of Extramural Research","doi-asserted-by":"publisher","award":["P30CA012197"],"award-info":[{"award-number":["P30CA012197"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006955","name":"Office of Extramural Research","doi-asserted-by":"publisher","award":["R01 DK080327"],"award-info":[{"award-number":["R01 DK080327"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006955","name":"Office of Extramural Research","doi-asserted-by":"publisher","award":["UL1 TR001998"],"award-info":[{"award-number":["UL1 TR001998"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Glucose tolerance (GT) is a major effector for adipose tissue (AT) remodeling in obesity, yet its molecular mechanisms remain incompletely defined. We hypothesized that the biophysical and molecular profiles of AT\u2010derived small extracellular vesicles (sEV\n                      <jats:sup>AT<\/jats:sup>\n                      ) change in response to glucose availability and differ by GT status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      sEV\n                      <jats:sup>AT<\/jats:sup>\n                      were isolated from plasma of individuals with normal GT (NGT) and impaired GT (IGT) (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095\/group) at fasting (0\u2009h) and 1\u2009h\u00a0post glucose challenge during oral glucose tolerance test (OGTT). sEV\n                      <jats:sup>AT<\/jats:sup>\n                      were characterized for size, concentration, surface expression of insulin receptor\u2010\u03b1 (INSR\u03b1), proteome, and insulin signaling\u2010related miRNAs. C2C12 myotubes were treated with sEV\n                      <jats:sup>AT<\/jats:sup>\n                      for 48\u2009h, followed by quantification of 84 insulin signaling\u2010related genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      The size and concentration of sEV\n                      <jats:sup>AT<\/jats:sup>\n                      did not differ between groups. At fasting, INSR\u03b1 expression on sEV\n                      <jats:sup>AT<\/jats:sup>\n                      was comparable; however, groups exhibited opposite directional changes at 1\u2010h OGTT. LC\u2013MS\/MS identified significant proteomic differences between NGT and IGT sEV\n                      <jats:sup>AT<\/jats:sup>\n                      . miR\u201027a\u20105p and miR\u2010145a\u20105p levels in sEV\n                      <jats:sup>AT<\/jats:sup>\n                      also differed significantly by GT status. Notably, treatment with sEV\n                      <jats:sup>AT<\/jats:sup>\n                      (IGT\u20100\u2009h) significantly downregulated insulin signaling\u2010related genes in myotubes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Distinct molecular signatures in sEV\n                      <jats:sup>AT<\/jats:sup>\n                      offer a unique insight into AT dysfunction during IGT and offer novel diagnostic and therapeutic targets.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/oby.70157","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T05:26:16Z","timestamp":1771824376000},"page":"896-907","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adipose Tissue\u2010Derived Small Extracellular Vesicles in Plasma Reveal Molecular Circuitries Underlying Glucose Intolerance"],"prefix":"10.1002","volume":"34","author":[{"given":"Shalini","family":"Mishra","sequence":"first","affiliation":[{"name":"Section of Gerontology and Geriatric Medicine, Department of Internal Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixin","family":"Su","sequence":"additional","affiliation":[{"name":"Section of Gerontology and Geriatric Medicine, Department of Internal Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Kumar","sequence":"additional","affiliation":[{"name":"Section of Gerontology and Geriatric Medicine, Department of Internal Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangeeta","family":"Singh","sequence":"additional","affiliation":[{"name":"Section of Gerontology and Geriatric Medicine, Department of Internal Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian S.","family":"Finlin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Endocrinology University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang\u2010Chi","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Data Science Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"},{"name":"Atrium Health Wake Forest Baptist Comprehensive Cancer Center Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Atrium Health Wake Forest Baptist Comprehensive Cancer Center Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"},{"name":"Section of Molecular Medicine, Department of Internal Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina M.","family":"Furdui","sequence":"additional","affiliation":[{"name":"Atrium Health Wake Forest Baptist Comprehensive Cancer Center Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"},{"name":"Section of Molecular Medicine, Department of Internal Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip 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