{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:13:23Z","timestamp":1787505203638,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100001583","name":"Diabetes Action Research and Education Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001583","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We aimed to examine the effect of obesity on the plasma free fatty acid (FFA) carrier proteome in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From 6 to 20\u2009weeks of age, male and female C57BL\/6J wild\u2010type mice were fed either a low\u2010fat diet (10% of kilocalories from fat) or a high\u2010fat diet (60% of kilocalories from fat), and leptin\u2010deficient ob\/ob mice were fed a low\u2010fat diet. Plasma FFA carrier proteins were isolated using a fatty acid pulldown assay and detected by proteomics. Plasma proteins and lipoprotein particles were separated by fast protein liquid chromatography (FPLC). Hepatic gene expression was quantified by qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In each sex, 284 FFA carrier proteins were detected, with dozens of differentially abundant proteins between lean and obese groups. We identified eight proteins that exhibited altered abundance in both models of obesity for both sexes, with the majority transcribed in the liver. Interestingly, proteins with lower or higher abundance in the plasma FFA carrier proteome in obesity showed correspondingly altered gene expression in the liver. Furthermore, FFAs coeluted with lipoprotein particles in FPLC fractions, with accentuated FFA levels in the low\u2010density lipoprotein fractions in obesity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Obesity remodels the spectrum of plasma FFA carriers, potentially via changes in gene expression and lipoprotein abundance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/oby.70214","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T02:30:41Z","timestamp":1778034641000},"page":"1300-1313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Plasma Free Fatty Acid Carrier Proteome is Altered in Diet\u2010Induced and Genetically Obese 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