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However, weight loss can lead to reductions in lean soft tissue mass and strength, compromising body composition and functional ability. Thus, identifying molecular predictors of muscular strength preservation during weight loss is critical to mitigating these effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a secondary analysis of the CALERIE trial, a 2\u2010year randomized controlled study of caloric restriction or ad\u00a0libitum intake in healthy adults without obesity. Changes in whole\u2010body mass and knee extensor strength were assessed over the first 12\u2009months of the study. Transcriptomic profiling was conducted in a subset who provided skeletal muscle samples. The relationship between strength change and gene expression change was modeled, controlling for whole\u2010body mass change.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Transcriptomics analysis on a subset of 42 individuals revealed 151 genes significantly associated with change in strength after accounting for change in whole\u2010body mass, including\n                      <jats:italic>HSP90AA1<\/jats:italic>\n                      and\n                      <jats:italic>EIF3A<\/jats:italic>\n                      . Pathway analyses revealed significant involvement of pathways related to cellular proliferation, immune regulation, and protein secretion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings highlight molecular pathways and genes supporting strength retention during caloric restriction\u2010induced weight loss.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      identifier: NCT00427194\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.clinicaltrials.gov\">www.clinicaltrials.gov<\/jats:ext-link>\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/oby70293-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/oby.70293","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T10:30:37Z","timestamp":1788258637000},"page":"1892-1903","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biological Pathways of Strength Preservation During Calorie Restriction\u2010Induced Weight Loss Among Adults Without Obesity"],"prefix":"10.1002","volume":"34","author":[{"given":"Nadia","family":"Moore","sequence":"first","affiliation":[{"name":"Duke Molecular Physiology Institute, Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9033-4116","authenticated-orcid":false,"given":"Akshay","family":"Bareja","sequence":"additional","affiliation":[{"name":"Duke Molecular Physiology Institute, Duke University School of Medicine  Durham North Carolina USA"},{"name":"Division of Endocrinology, Metabolism, and Nutrition, Department of Medicine Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1407-1622","authenticated-orcid":false,"given":"Leanna 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