{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:02:14Z","timestamp":1790845334747,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The insulin receptor (IR) and the insulin\u2010like growth factor I receptor (IGF\u2010IR) regulate adipose tissue (AT) differentiation. This study investigated the expression of IGF\u2010IR, IR, and the IR isoforms (IRa and IRb) in human subcutaneous (S\u2010ASC) and visceral (V\u2010ASC) adipose\u2010derived stem cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>S\u2010AT and V\u2010AT biopsies were obtained from human donors and ASC were differentiated into adipocytes. mRNA levels of all receptors were quantified by qPCR at baseline and after differentiation. IR protein expression and adipogenic efficiency were assessed by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In undifferentiated ASC, IGF\u2010IR transcripts exceeded IR and were higher in S\u2010ASC than V\u2010ASC. Upon differentiation, IGF\u2010IR decreased in S\u2010ASC but remained unchanged in V\u2010ASC, whereas IR increased in both depots. S\u2010ASC displayed ~twofold higher IRa expression and greater insulin\u2010induced Akt activation. During adipogenesis, IR expression rose in both depots, with IRb exceeding IRa particularly in V\u2010ASC (+50%). In whole AT, IR exceeded IGF\u2010IR, and no depot\u2010specific differences were observed between IR isoforms. Adipogenic conversion was higher in S\u2010ASC (60%) than V\u2010ASC (20%). Only ~77% of S\u2010ASC\u2010 and ~61% of V\u2010ASC\u2010derived adipocytes expressed surface IR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Depot\u2010specific dynamics of IR isoforms may influence adipogenic capacity and insulin responsiveness and suggest partially receptor\u2010independent pathways of adipocyte differentiation.<\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/oby70315-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/oby.70315","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:41:20Z","timestamp":1790844080000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Depot\u2010Specific Insulin Receptor Isoform Dynamics During Human Adipose\u2010Derived Stem Cell Adipogenesis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8188-9638","authenticated-orcid":false,"given":"Valentina Annamaria","family":"Genchi","sequence":"first","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area\u2010Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases University of Bari Aldo Moro  Bari 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Regenerative Medicine and Ionian Area\u2010Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases University of Bari Aldo Moro  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6587-6087","authenticated-orcid":false,"given":"Nicola","family":"Marrano","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area\u2010Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases University of Bari Aldo Moro  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9527-9048","authenticated-orcid":false,"given":"Giuseppina","family":"Biondi","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area\u2010Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases University of Bari Aldo Moro  Bari 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