{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T12:09:23Z","timestamp":1770466163090,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,1,29]],"date-time":"2023-01-29T00:00:00Z","timestamp":1674950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Angiogenesis is indeed a vital process in the progression of carcinomas, including that of larynx. Therefore, this study (AngLaC) aimed to identify candidate angiogenesis\u2010related biomarkers in laryngeal carcinoma patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Prospective controlled cohort study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Tertiary referral center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>In silico<\/jats:italic> analyses of angiogenesis\u2010related genes in laryngeal carcinoma were performed to determine candidate biomarkers. Serum levels of candidate biomarkers were determined via enzyme\u2010linked immunosorbent assay in laryngeal carcinoma patients as well as in an age and gender\u2010matched control group. The associations of the biomarkers with clinical parameters were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study included 60 laryngeal carcinoma patients and 20 healthy controls. The serum levels of osteopontin, IGFBP\u20103, VEGF, sVEGFR\u20101, and VEGFR\u20102 were significantly higher in the patient group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001, <jats:italic>p<\/jats:italic>\u2009\u2264\u2009.001, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01, respectively). High osteopontin and sVEGFR\u20101 levels were associated with locoregional\u2010recurrence (<jats:italic>p<\/jats:italic>\u2009=\u2009.024, <jats:italic>p<\/jats:italic>\u2009=\u2009.016, respectively). IGFBP\u20103 had the highest diagnostic sensitivity (81.4%) and specificity (80%) among the molecules that were investigated (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). High sVEGFR\u20101 and low VEGFR\u20102 levels were associated with poor overall\u2010survival (<jats:italic>p<\/jats:italic>\u2009=\u2009.037, <jats:italic>p<\/jats:italic>\u2009=\u2009.027, respectively). High osteopontin and sVEGFR\u20101 levels were associated with poor disease\u2010specific survival rates (<jats:italic>p<\/jats:italic>\u2009=\u2009.035, <jats:italic>p<\/jats:italic>\u2009=\u2009.018, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>High serum levels of sVEGFR\u20101 and osteopontin as well as low serum levels of VEGFR\u20102 proved to be poor prognostic in terms of survival in laryngeal carcinoma. VEGF, sVEGFR1, VEGFR2, IGFBP\u20103, and osteopontin levels were found to be significantly increased in larynx cancer patients compared to the normal population. Further studies on osteopontin and sVEGFR\u20101 are required in order to determine their associations with recurrence.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ohn.219","type":"journal-article","created":{"date-parts":[[2023,1,29]],"date-time":"2023-01-29T10:45:05Z","timestamp":1674989105000},"page":"1433-1442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Candidate Angiogenesis\u2010Related Biomarkers in Patients with Laryngeal Carcinoma (AngLaC): A Prospective Cohort Study"],"prefix":"10.1002","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8813-0357","authenticated-orcid":false,"given":"A. Erim","family":"Pamuk","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Hacettepe University School of Medicine, Sihhiye  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Emre","family":"Gedik","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology Hacettepe University Cancer Institute, Sihhiye  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilda","family":"Sutay Suslu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Hacettepe University School of Medicine, Sihhiye  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6751-2217","authenticated-orcid":false,"given":"Gurcan","family":"Gunaydin","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology Hacettepe University Cancer Institute, Sihhiye  Ankara 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