{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:07:46Z","timestamp":1790348866577,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Advancements in tissue\u2010engineered tracheal replacement (TETR) show promise for the use of partially decellularized tracheal grafts (PDTG) to address critical gaps in airway management and reconstruction. In this study, aiming to leverage the immunoprivileged nature of cartilage to preserve tracheal biomechanics, we optimize PDTG for retention of native chondrocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Comparison in vivo murine study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Research Institute affiliated with Tertiary Pediatric Hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PDTG were created per a shortened decellularization protocol using sodium dodecyl sulfate, then biobanked via cryopreservation technique. Decellularization efficiency was characterized by DNA assay and histology. Viability and apoptosis of chondrocytes in preimplanted PDTG and biobanked native trachea (control) was assessed with live\/dead and apoptosis assays. PDTG (N\u2009=\u20095) and native trachea (N\u2009=\u20096) were orthotopically implanted in syngeneic recipients for 1\u2010month. At the endpoint, microcomputed tomography (micro\u2010CT) was employed to interrogate graft patency and radiodensity in vivo. Vascularization and epithelialization were qualitatively analyzed using histology images following explant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PDTG exhibited complete decellularization of all extra\u2010cartilaginous cells and reduced DNA content compared to control. Chondrocyte viability and nonapoptotic cell populations were improved utilizing biobanking and shorter decellularization time. All grafts remained patent. Evaluation of graft radiodensity at 1 month revealed elevation of Hounsfield units in both PDTG and native compared to host, with PDTG showing higher radiodensity than native. PDTG supported complete epithelialization and functional reendothelialization 1\u2010month postimplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Optimizing PDTG chondrocyte viability is a key component to successful tracheal replacement. Ongoing research seeks to evaluate the acute and chronic immunogenicity of PDTG.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ohn.404","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T06:16:45Z","timestamp":1686723405000},"page":"1241-1246","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Optimization of Chondrocyte Viability in Partially Decellularized Tracheal Grafts"],"prefix":"10.1002","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-0484","authenticated-orcid":false,"given":"Maxwell","family":"Bergman","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery The Ohio State University Medical Center Columbus Ohio USA"},{"name":"Department of Pediatric Otolaryngology Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Harwood","sequence":"additional","affiliation":[{"name":"Department of Pediatric Otolaryngology Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lumei","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayali","family":"Dharmadikhari","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly M.","family":"Shontz","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tendy","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Otolaryngology Nationwide Children's Hospital  Columbus Ohio USA"},{"name":"Center for Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599816628486"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.129.5.523"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.03.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489419826134"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0583"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2019.107421"},{"issue":"5","key":"e_1_2_9_8_1","first-page":"577","article-title":"Vascular grafts and valves that animate, made from decellularized biologically\u2010engineered tissue tubes","volume":"61","author":"Syedain ZH","year":"2020","journal-title":"J Cardiovasc Surg (Torino)"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489416656646"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30206"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/20417314211017417"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21981"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(00)01854-3"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.5.3.9494"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(96)70366-5"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2015.03.005"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11748-004-0101-4"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(00)01705-7"},{"key":"e_1_2_9_19_1","first-page":"A7601","article-title":"The limit of lung ischemia in mouse lung transplantation: 72 hours at 0\u00b0C or 3 hours at 37\u00b0C. New players in lung injury and repair","volume":"197","author":"Wang X","year":"2018","journal-title":"Am Thorac Soc"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.11.008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.28781"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002480-200201000-00006"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-108-6_1"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ohn.404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:01:45Z","timestamp":1760968905000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1002\/ohn.404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,14]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/ohn.404"],"URL":"https:\/\/doi.org\/10.1002\/ohn.404","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"value":"0194-5998","type":"print"},{"value":"1097-6817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,14]]},"assertion":[{"value":"2022-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}