{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:03:16Z","timestamp":1790247796509,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Cisplatin is a chemotherapeutic agent with the undesirable side effect of ototoxicity. Transtympanic injections of antioxidant formulations may provide local otoprotection. We tested a novel antioxidant\u2010eluting microparticle for its otoprotective capability from systemic cisplatin as measured by cochlear electrophysiology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Basic science.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Translational research laboratory.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Eighteen mice were assigned to three groups. All mice underwent baseline click\u2010evoked auditory brainstem response (ABR) audiometry and right ear microparticle injections before beginning 42\u2010day intraperitoneal administration regimens of either saline (healthy control empty microparticle [HCEMP] group) or cisplatin (cisplatin empty microparticle [CEMP] group and cisplatin\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine microparticle [CNAC] group). These regimens consisted of three 4\u2010day cycles of intraperitoneal saline or cisplatin administration followed by 10 rest days. HCEMP and CEMP received right\u2010sided transtympanic empty microparticles, and CNAC received transtympanic\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine eluting microparticles. On day 43, all mice underwent posttreatment ABR. ABR thresholds and threshold shifts were analyzed with mixed\u2010effects models and Tukey's post hoc tests and were compared across pretreatment\/posttreatment ears, treatment groups, and injected and non\u2010injected ears.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that threshold shifts in the ears that received a transtympanic injection of\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine and three cycles of intraperitoneal cisplatin were similar to the paired ears of mice that received no cisplatin. Mice that received a transtympanic injection without\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine and intraperitoneal cisplatin had increased thresholds compared to mice that received a transtympanic injection of\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine and cisplatin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Transtympanic\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine microparticle injections provided functional otoprotection in cisplatin\u2010exposed mice.\n                    <\/jats:p>\n                  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