{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T19:28:13Z","timestamp":1783020493365,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Human papillomavirus (HPV) accounts for the majority of oropharyngeal squamous cell carcinoma (OPSCC) cases in the United States but understanding the prevalence of high\u2010risk HPV in oropharyngeal tissue remains poor. We evaluated nanopore sequencing, a novel rapid, and long\u2010read sequencing platform, on OPSCC tissue and tested it in benign tonsils from pediatric and young adult patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Cross\u2010sectional study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Academic tertiary referral center.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Formalin\u2010fixed, paraffin\u2010embedded (FFPE) specimens of HPV\u2010positive and negative OPSCC diagnosed from 2013 to 2023 were sequenced on the nanopore MinION platform using an amplicon\u2010based approach. Nanopore sequencing was subsequently performed on FFPE tissue from benign tonsillectomy in patients ages 30 and younger performed from 2016 to 2023.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In 54 OPSCC cases, nanopore sequencing detected HPV DNA in all 27 HPV\u2010positive specimens with 96% of cases exhibiting high abundance (HPV reads &gt;1000). HPV DNA was also detectable in all 27 HPV\u2010negative specimens, but 89% had a low abundance. By interpreting high HPV abundance as HPV\u2010positive, nanopore sequencing demonstrated a 96% sensitivity, 89% specificity, 90% positive predictive value, and 96% negative predictive value rate. In 150 benign tonsillectomy cases, 27% had HPV DNA detected by nanopore sequencing, all in low abundance, including 37% of patients younger than 10 years old. HPV DNA sequences detected belonged to subtype 16.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Nanopore sequencing demonstrated high sensitivity and specificity for HPV DNA in FFPE OPSCC tissue. High\u2010risk HPV prevalence in pediatric and young adults may be higher than previously studied.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ohn.70240","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T13:09:36Z","timestamp":1776172176000},"page":"136-144","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nanopore Sequencing for HPV in Oropharyngeal Squamous Cell Carcinoma and Benign Tonsil Specimens"],"prefix":"10.1002","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3644-6800","authenticated-orcid":false,"given":"Mikayla G.","family":"Hubbard","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang\u2010Sheng","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Emerson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Schreiber","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1809-7819","authenticated-orcid":false,"given":"Diane W.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-19-0038"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pvr.2017.05.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00603-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0912217"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-017-0484-y"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21388"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.21.01752"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2019.11.021"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2023.1573"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-018-0255-1"},{"key":"e_1_2_9_12_1","unstructured":"Committee APED. Position statement on oral HPV screening in the US population; 2017. 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