{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:10:22Z","timestamp":1789153822080,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To perform a systematic review and meta\u2010analysis on the utility of circulating tumor DNA (ctDNA) in serum as a biomarker for post\u2010treatment surveillance of human papillomavirus (HPV)\u2010positive oropharyngeal squamous cell carcinoma (OPSCC), given the improved treatment response and 10% to 20% recurrence rate in this patient population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Data Sources<\/jats:title>\n                    <jats:p>Articles were sourced from PubMed. Studies were excluded if they focused on HPV\u2010driven cancers in other anatomical regions, lacked pretreatment and posttreatment HPV viral load data, or were published more than 20 years ago. All literature available in English was considered.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Review Methods<\/jats:title>\n                    <jats:p>A PRISMA\u2010compliant review identified 419 articles, and 20 studies met all inclusion criteria. Data extraction and quality assessment were performed using Oxford Centre for Evidence\u2010Based Medicine and Newcastle\u2010Ottawa Scale criteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Across 3505 clinically correlated tests, pooled sensitivity was 80.3% (95% CI 70.9%\u201087.2%) and specificity was 95.5% (90.9%\u201097.8%). Meta\u2010analysis of our studies found the overall prevalence of recurrent HPV\u2009+\u2009OPSCC after curative treatment was 9.70% (95% CI 8.72%\u201010.78%). Negative Predictive Value (NPV) at this prevalence was 97.7% (96.7%\u201098.5%) and Positive Predictive Value (PPV) was 66.0% (49.5%\u201080.3%). A positive test for ctDNA after curative treatment had a 130.3 (95% CI 56.60\u2010298.71) fold higher odds of disease recurrence compared to a negative test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CtDNA is a promising biomarker for early detection of recurrence. Standardized protocols and quantitative thresholds are needed to fully establish its role in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ohn.70426","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:45:22Z","timestamp":1789152322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recurrence and Circulating Tumor DNA in Human Papillomavirus\u2010Positive Oropharyngeal Cancer: A Meta\u2010Analysis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2393-5808","authenticated-orcid":false,"given":"Claire","family":"Sturek","sequence":"first","affiliation":[{"name":"Department of Graduate Medical Education Walter Reed National Military Medical Center Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Carr","sequence":"additional","affiliation":[{"name":"Department of Surgery Uniformed Services University Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Thornton","sequence":"additional","affiliation":[{"name":"Department of Surgery Walter Reed National Military Medical Center Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8477-975X","authenticated-orcid":false,"given":"Michael","family":"Orestes","sequence":"additional","affiliation":[{"name":"Department of Surgery Uniformed Services University Bethesda Maryland USA"},{"name":"Department of Surgery Walter Reed National Military Medical Center Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2020.08.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2023.104184"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-23-1064"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.02444"},{"key":"e_1_2_9_6_1","unstructured":"National Comprehensive Cancer Network.NCCN Clinical Practice Guidelines in Oncology: Head and Neck Cancers. 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