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However, its uptake mechanism and its specificity to various cancer cell lines have been less studied. Herein, we tested ATSM\u2212Cu(II) toxicity, uptake, and reduction, using four different cell types: (1) mouse breast cancer cells (DA\u20103), (2) human embryonic kidney cells (HEK\u2010293), (3) breast cancer cells (MCF\u20107), and (4) cervical cancer cells (Hela) under normoxic and hypoxic conditions. We showed that ATSM\u2212Cu(II) is toxic to breast cancer cells under normoxic and hypoxic conditions; however, it is not toxic to normal HEK\u2010293 non\u2010cancer cells. We showed that the Cu(I) content in breast cancer cell after treatment with ATSM\u2212Cu(II) under hypoxic conditions is higher than in normal cells, despite that the uptake of ATSM\u2212Cu(II) is a bit higher in normal cells than in breast cancer cells. This study suggests that the redox potential of ATSM\u2212Cu(II) is higher in breast cancer cells than in normal cells; thus, its toxicity to cancer cells is increased.<\/jats:p>","DOI":"10.1002\/open.202100044","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T16:15:09Z","timestamp":1619626509000},"page":"486-492","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cellular Uptake of the ATSM\u2212Cu(II) Complex under Hypoxic Conditions"],"prefix":"10.1002","volume":"10","author":[{"given":"Gulshan R.","family":"Walke","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Exact Sciences, and the Institute for Nanotechnology and advanced materials (BINA) Bar-Ilan University  5290002 Ramat-Gan Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelly","family":"Meron","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Exact Sciences, and the Institute for Nanotechnology and advanced materials (BINA) Bar-Ilan University  5290002 Ramat-Gan Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia","family":"Shenberger","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Exact Sciences, and the Institute for Nanotechnology and advanced materials (BINA) Bar-Ilan University  5290002 Ramat-Gan Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lada","family":"Gevorkyan\u2010Airapetov","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Exact Sciences, and the Institute for Nanotechnology and advanced materials (BINA) Bar-Ilan University  5290002 Ramat-Gan Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1741-6892","authenticated-orcid":false,"given":"Sharon","family":"Ruthstein","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Exact Sciences, and the Institute for Nanotechnology and advanced materials (BINA) Bar-Ilan University  5290002 Ramat-Gan Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,28]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2015.02.006"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553000601002324"},{"key":"e_1_2_7_4_1","first-page":"1408","volume":"58","author":"Brown J. 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