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In this work, <jats:italic>p<\/jats:italic>\u2010xylene and toluene are introduced as greener alternates to dichloromethane (DCM) for preparing protected peptide amide fragments from a Sieber amide resin. The <jats:italic>N\u2010<\/jats:italic>dealkylation is a demanding chemical reaction, requiring that the cleavage protocol be optimised to ensure complete cleavage from the resin. After a 30\u2005min reaction time, only 66\u2009% of the final peptide product was retrieved even with the conventional dichloromethane solvent. Therefore, 120\u2005min was considered sufficient to fully cleave the peptide from the Sieber amide resin. This work reaffirms the fact that greening strategies are far from detrimental, with green alternatives often outperforming their replaced counterparts.<\/jats:p>","DOI":"10.1002\/open.202200236","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T22:17:26Z","timestamp":1671833846000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Greener Cleavage of Protected Peptide Fragments from Sieber Amide Resin"],"prefix":"10.1002","volume":"11","author":[{"given":"Othman","family":"Al Musaimi","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4647-444X","authenticated-orcid":false,"given":"Varshitha","family":"Gavva","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-5903","authenticated-orcid":false,"given":"Daryl R.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,23]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15020222"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02697"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph11020042"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph12020052"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40580-018-0170-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202101465"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1GC04387K"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00897a025"},{"key":"e_1_2_9_9_1","first-page":"59","volume-title":"Peptide-Based Drug Discovery: Challenges and New Therapeutics","author":"Jad Y. 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