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In this study, a series of polyacrylates with hydrophobic physical crosslinking and chemical crosslinking were prepared using precipitation polymerization, and their structures were characterized by nuclear magnetic resonance spectroscopy and Fourier\u2010transform infrared spectroscopy. The optimal reaction conditions were determined according to the effect of reaction time, azodiisobutyronitrile, Span 60, allyl pentaerythritol, and octadecyl methacrylate (OMA) contents on the viscosity of the polyacrylate microgel, combined with the effects of allyl pentaerythritol and OMA contents on the subcutaneous immune safety of the polyacrylate microgel in BALB\/c mice. The polyacrylate microgels with different OMA contents showed good biological safety. In addition, <jats:italic>in\u2005vivo<\/jats:italic> immunity experiments were carried out in mice to analyze the adjuvant properties of ovalbumin as a model antigen. Based on the titer results of the IgG1 and IgG2a antibodies, with 1\u2005wt\u2009% OMA content, the polyacrylate microgel vaccine could optimally induce the body to produce an immune response type dominated by Th2\u2010type humoral immune response and supplemented by Th1\u2010type cellular immune response.<\/jats:p>","DOI":"10.1002\/open.202200246","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T07:56:26Z","timestamp":1680508586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preparation and Evaluation of Polyacrylate Microgels and Their Adjuvant Activities Using Ovalbumin as a Model Antigen"],"prefix":"10.1002","volume":"12","author":[{"given":"Chengyu","family":"Li","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering Hebei University of Science and Technology  050018 Shijiazhuang Hebei Province P. R. 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