{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:01:04Z","timestamp":1791172864272,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T00:00:00Z","timestamp":1707091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tetracyclines are a class of commonly used four\u2010ringed antibiotics. A series of DNA aptamers were recently obtained using the capture\u2010SELEX (systematic evolution of ligands by exponential enrichment) method to bind to oxytetracycline, and one of the aptamers can bind to a few other tetracycline antibiotics as well. Upon binding to the aptamers, the intrinsic fluorescence of tetracycline antibiotics can be enhanced. At least 10 different DNA aptamers were isolated from the previous selection experiment. In this work, a systematic characterization of these ten aptamers was performed. Each of these aptamers shows a different degree of fluorescence enhancement ranging from around 1\u2010fold to over 20\u2010fold. Fluorescence enhancement was boosted in the presence of Mg<jats:sup>2+<\/jats:sup>. Isothermal titration calorimetry (ITC) studies were done and showed a great variety in dissociation constant (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>) from 62\u2005nM to 1.6\u2005\u03bcM. Steady\u2010state fluorescence spectroscopy and fluorescence lifetime studies showed a correlation between fluorescence lifetime and degree of fluorescence enhancement. A few aptamers showed slow binding kinetics, although no correlation was found between the kinetics of fluorescence change and degree of fluorescence enhancement. This is the first study of ten different aptamers for the same target, providing fundamental insights into aptamer binding and bioanalytical applications.<\/jats:p>","DOI":"10.1002\/open.202300250","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T01:52:13Z","timestamp":1707270733000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of the Binding Properties of Ten Aptamers Using the Intrinsic Fluorescence of Oxytetracycline"],"prefix":"10.1002","volume":"13","author":[{"given":"Yichen","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Ontario N2L 3G1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biwen","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Ontario N2L 3G1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5918-9336","authenticated-orcid":false,"given":"Juewen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Ontario N2L 3G1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,5]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050339"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios11070232"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09298.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios8020054"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c01140"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202008663"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-017-0076"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(01)00063-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.10.073"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.06.033"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3AN00154G"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c01561"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjc-2022-0253"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3AN01368E"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios12090717"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl347"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c04349"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/b304523b"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac2026699"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0cp00044b"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.0c00586"}],"container-title":["ChemistryOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/open.202300250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:32:39Z","timestamp":1760967159000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/open.202300250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,5]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/open.202300250"],"URL":"https:\/\/doi.org\/10.1002\/open.202300250","archive":["Portico"],"relation":{},"ISSN":["2191-1363","2191-1363"],"issn-type":[{"value":"2191-1363","type":"print"},{"value":"2191-1363","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,5]]},"assertion":[{"value":"2023-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202300250"}}