{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T06:21:56Z","timestamp":1778134916379,"version":"3.51.4"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:p>\n                    Recognizing the therapeutic value of the Genus\n                    <jats:italic>Fraxinus<\/jats:italic>\n                    worldwide, this study evaluates the antioxidant potential of\n                    <jats:italic>Fraxinus hookeri Wenz.<\/jats:italic>\n                    (\n                    <jats:italic>F. hookeri<\/jats:italic>\n                    ) against CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced nephrotoxicity in rats. Forty\u2010eight rats are randomly allocated into eight groups (six rats each). Antioxidant enzymes, genotoxicity, urine and serum markers, and tissue histopathology are assessed to determine their nephroprotective effects. The Control group remains untreated, while the DMSO group receives vehicle olive oil intraperitoneally and DMSO orally (3 ml\/kg). All other groups, except Control and DMSO, are given CCl\n                    <jats:sub>4<\/jats:sub>\n                    (3 ml\/kg, i.p., in 30% olive oil) twice weekly for 4 weeks. The CCl\n                    <jats:sub>4<\/jats:sub>\n                    group receives only CCl\n                    <jats:sub>4<\/jats:sub>\n                    . The Rutin group receives reference drug Rutin orally (50 mg\/kg). MEFH100 and MEFH200 groups are given MEFH at 100 and 200 mg\/kg, respectively, and NHFH100 and NHFH200 receive NHFH at the same doses. Rutin and\n                    <jats:italic>F. hookeri<\/jats:italic>\n                    treatment effectively (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) restore urine and serum markers disrupted by CCl\n                    <jats:sub>4<\/jats:sub>\n                    . CCl\n                    <jats:sub>4<\/jats:sub>\n                    reduced (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05) antioxidant enzymes (CAT, SOD, POD) and increased TBAR levels and DNA damage, which are reversed by cotreatment with\n                    <jats:italic>F. hookeri<\/jats:italic>\n                    and Rutin. Histopathological improvements (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) are also observed with\n                    <jats:italic>F. hookeri.<\/jats:italic>\n                    The results indicate that\n                    <jats:italic>F. hookeri<\/jats:italic>\n                    enhances antioxidant defenses, supporting its potential against CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced nephrotoxicity.\n                  <\/jats:p>","DOI":"10.1002\/open.202400515","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T04:51:53Z","timestamp":1750049513000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nephroprotective Effects of\n                    <i>Fraxinus Hookeri Wenz.<\/i>\n                    Against Renal Toxicity and DNA Oxidative Damages Induced by CCl\n                    <sub>4<\/sub>\n                    in Rats"],"prefix":"10.1002","volume":"14","author":[{"given":"Raheela","family":"Sarwar","sequence":"first","affiliation":[{"name":"Center of Biotechnology and Microbiology University of Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bashir","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Center of Biotechnology and Microbiology University of Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayman M.","family":"Al\u2010Qaaneh","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences Al\u2010Balqa Applied University (BAU)  Al\u2010Salt 19117 Jordan"},{"name":"Department of Pharmaceutical Technology Faculty of Pharmacy Jordan University of Science and Technology (JUST)  Irbid 22110 Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumaila","family":"Rauf","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laiba","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Khyber Teaching Hospital Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mounir M.","family":"Bekhit","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics College of Pharmacy King Saud University  PO Box 2457 Riyadh 11451 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Said","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Health and Biological Sciences Abasyn University Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6089-8906","authenticated-orcid":false,"given":"Nadeem","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Biology Science Faculty Selcuk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arshad","family":"Farid","sequence":"additional","affiliation":[{"name":"Gomal Center of Biochemistry and Biotechnology Gomal University  D. 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