{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T19:39:00Z","timestamp":1788118740619,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,22]],"date-time":"2025-06-22T00:00:00Z","timestamp":1750550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,22]],"date-time":"2025-06-22T00:00:00Z","timestamp":1750550400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2025,9]]},"abstract":"\n Plasma\u2010generated H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n can be used to fuel biocatalytic reactions that require H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n as a cosubstrate, such as the conversion of ethylbenzene to (\n R<\/jats:italic>\n )\u20101\u2010phenylethanol ((\n R<\/jats:italic>\n )\u20101\u2010PhOl) catalyzed by unspecific peroxygenase from\n Agrocybe aegerita<\/jats:italic>\n (r\n Aae<\/jats:italic>\n UPO). Immobilization is recently shown to protect biocatalysts from inactivation by highly reactive plasma\u2010produced species; however, H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n supply by the employed plasma sources (\u03bcAPPJ and DBD) is limiting for r\n Aae<\/jats:italic>\n UPO performance. This study evaluates a recently introduced capillary plasma jet for suitability to supply H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n in\u2009situ. H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n production is modulated by varying the water concentration in the feed gas, providing a greater operating window for applications in plasma\u2010driven biocatalysis. In a static system after 80\u2009min of biocatalysis, a turnover number of 44,199\u2009mol\n \n (\n R<\/jats:italic>\n )\u20101\u2010PhOl\n <\/jats:sub>\n mol\n \u22121<\/jats:sup>\n \n r\n Aae<\/jats:italic>\n UPO\n <\/jats:sub>\n is achieved without significant enzyme inactivation. By exchanging the reaction solution every 5\u2009min, a total product yield of 122\u2009\u03bcmol (\n R<\/jats:italic>\n )\u20101\u2010PhOl is achieved in 700\u2009min run time, resulting in a total turnover number of 174,209\u2009mol\n \n (\n R<\/jats:italic>\n )\u20101\u2010PhOl\n <\/jats:sub>\n mol\n \u22121<\/jats:sup>\n \n r\n Aae<\/jats:italic>\n UPO\n <\/jats:sub>\n . This study concludes that the capillary plasma jet, due to its flexibility regarding feed gas, admixtures, and power input, is well suited for in\u2009situ H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n generation for plasma\u2010driven biocatalysis tailoring to enzymes with high H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n turnover.\n <\/jats:p>","DOI":"10.1002\/open.202500057","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T02:59:33Z","timestamp":1750647573000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Atmospheric Pressure Capillary Plasma Jet Is Well\u2010Suited to Supply H\n 2<\/sub>\n O\n 2<\/sub>\n for Plasma\u2010Driven Biocatalysis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7905-9456","authenticated-orcid":false,"given":"Tim","family":"Dirks","sequence":"first","affiliation":[{"name":"Applied Microbiology Faculty of Biology and 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Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2344-2146","authenticated-orcid":false,"given":"Judith","family":"Golda","sequence":"additional","affiliation":[{"name":"Plasma Interface Physics Faculty of Physics and Astronomy Ruhr University Bochum 44801 Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4100-8829","authenticated-orcid":false,"given":"Julia E.","family":"Bandow","sequence":"additional","affiliation":[{"name":"Applied Microbiology Faculty of Biology and Biotechnology Ruhr University Bochum 44801 Bochum 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