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It is primarily expressed in organs such as bones, liver, intestine, and placenta during pregnancy, playing a crucial role in cellular processes like gene expression, transport, and metabolism. Physiological ALP levels vary with age and sex, with normal serum ranges for healthy adults between 40 and 190 U\/L. Alterations in ALP levels can be indicative of several pathologies, including cancer diagnosis and metastasis, as well as bone growth dysfunctions and hypophosphatasia. Conventional methods for ALP detection often require complex assay principles, extensive sample pretreatment, and trained personnel. Herein, the development of a portable, flexible electrochemical sensor fabricated through screen\u2010printing to monitor ALP levels in biological samples is introduced. The flexible electrochemical sensor, characterized by high efficiency, sustainability, low cost, and ease of disposal, achieves detection limit as low as 0.03 and 0.08 U\/L, respectively, in buffer solution and human serum samples, and a satisfactory repeatability lower than 10%. This simple sensor configuration approach enables real\u2010time disease monitoring and improves access to point\u2010of\u2010care diagnostics, paving the way for affordable, decentralized sensors that support early diagnosis and better healthcare.<\/jats:p>","DOI":"10.1002\/open.202500113","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T02:22:31Z","timestamp":1744597351000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Flexible Screen\u2010Printed Electrochemical Sensor for Alkaline Phosphatase Detection in Biofluids for Biomedical Applications"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8287-059X","authenticated-orcid":false,"given":"Panagiota M.","family":"Kalligosfyri","sequence":"first","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7531-1159","authenticated-orcid":false,"given":"Antonella","family":"Miglione","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4481-396X","authenticated-orcid":false,"given":"Raghad","family":"Alhardan","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy Ankara University  06560 Ankara T\u00fcrkiye"},{"name":"The Graduate School of Health Sciences Ankara University  06110 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Iula","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iclal","family":"Atay","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy Ankara University  06560 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim A.","family":"Darwish","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University  P.O. Box 2457 Riyadh 11451 Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-7604","authenticated-orcid":false,"given":"Sevinc","family":"Kurbanoglu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy Ankara University  06560 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8274-7452","authenticated-orcid":false,"given":"Stefano","family":"Cinti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  80131 Naples Italy"},{"name":"Bioelectronics Task Force at University of Naples Federico II  Via Cinthia 21 80126 Naples Italy"},{"name":"Sbarro Institute for Cancer Research and Molecular Medicine Center for Biotechnology, College of Science and Technology Temple University  Philadelphia, Pennsylvania 19122 U. S. 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