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Here, the conjugated polymers derived from homogentisic acid (HGA), polyHGA (a simplified model of pyomelanin), formed in an ex\u2009vivo tendon model are examined with a view to understanding interactions between melanins and the extracellular matrix (ECM) using a selection of different analytical techniques, including spectroscopy (energy dispersive X\u2010ray, infrared, and Raman), X\u2010ray diffraction, and microscopy (electron, optical, and scanning electrochemical). The combination of techniques was used to facilitate an understanding of subtle differences in the composition and distribution of ECM components, hydroxyapatite, and melanin in the tendons for the first time. PolyHGA deposition in connective tissues in patients with alkaptonuria is a significant burden and causes multiple tendon ruptures due to the significant alterations in collagen properties. A similar pathology is seen in the wider population from calcific tendinitis because of hydroxyapatite crystal deposition in tendons of the shoulder and lower limbs, in particular.<\/jats:p>","DOI":"10.1002\/open.202500194","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T02:50:21Z","timestamp":1744599021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of Interactions Between Pyomelanin and the Extracellular Matrix in an Ex Vivo Turkey Tendon Model"],"prefix":"10.1002","volume":"14","author":[{"given":"Rebecca F.","family":"Shepherd","sequence":"first","affiliation":[{"name":"Lancaster Medical School Lancaster University  Lancaster LA1 4AT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanaa A.","family":"Galeb","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Science &amp; Arts College, Rabigh Campus King Abdulaziz University  Rabigh 25732 Saudi Arabia"},{"name":"Department of Chemistry Lancaster University  Lancaster LA1 4YB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade","family":"Bentham","sequence":"additional","affiliation":[{"name":"Lancaster Medical School Lancaster University  Lancaster LA1 4AT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Moshrefi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Memorial University of Newfoundland  45 Arctic Ave St. John's NL A1C 5S7 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katelyn","family":"Ryan","sequence":"additional","affiliation":[{"name":"Department of Chemistry Memorial University of Newfoundland  45 Arctic Ave St. John's NL A1C 5S7 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nur Adeelah Che Ahmad","family":"Tantowi","sequence":"additional","affiliation":[{"name":"Lancaster Medical School Lancaster University  Lancaster LA1 4AT UK"},{"name":"Department of Economics and Agribusiness School of Economics, Finance and Banking Universiti Utara Malaysia  06010 Sintok Kedah Darul Aman Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara J.","family":"Baldock","sequence":"additional","affiliation":[{"name":"Department of Chemistry Lancaster University  Lancaster LA1 4YB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan R.","family":"Halcovitch","sequence":"additional","affiliation":[{"name":"Department of Chemistry Lancaster University  Lancaster LA1 4YB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7811-8840","authenticated-orcid":false,"given":"T. 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