{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:26:05Z","timestamp":1764998765519,"version":"3.46.0"},"reference-count":66,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>\n                    A systematic investigation of the catalyst\u2010free Strecker reaction is conducted in aqueous buffer, offering an efficient and green alternative that leads to \u03b1\u2010aminonitriles without the need of chromatography. Optimization reveals that low pH and high buffer concentration significantly enhance conversion, with yields up to 97%. Broad substrate scope is demonstrated with various aldehydes, ketones, and amines, leading to key intermediates for natural and unnatural amino acids. Potassium cyanide and acetone cyanohydrin are established, latter as a safer and effective cyanide source, and reactions are conducted also in buffer\u2013methyl\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl ether mixed solvent further improving the methodology. It is hypothesized that, based on the similarity to cyanohydrin formation, the hydroxynitrile lyases (HNLs) might transform imines to aminonitriles. Adding AtHNL and HbHNL further accelerates the reaction suggesting an undiscovered reactivity of these enzymes.\n                  <\/jats:p>","DOI":"10.1002\/open.202500389","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T06:22:31Z","timestamp":1758522151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Buffer\u2010Mediated Catalyst\u2010Free Strecker Reaction Toward Enzymatic Implementation"],"prefix":"10.1002","volume":"14","author":[{"given":"P\u00e9ter","family":"Magyar","sequence":"first","affiliation":[{"name":"Medicinal Chemistry Research Group HUN\u2010REN Research Centre for Natural Sciences  Magyar tud\u00f3sok k\u00f6r\u00fatja 2 Budapest 1117 Hungary"},{"name":"Department of Organic Chemistry and Technology Faculty of Chemical Technology and Biotechnology Budapest University of Technology and Economics  M\u0171egyetem rkp. 3 Budapest 1111 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