{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:25:55Z","timestamp":1786940755127,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    <jats:italic>Anchusa azurea<\/jats:italic>\n                    has attracted increasing interest as a medicinal plant due to its phenolic and flavonoid compounds, which may contribute to its antioxidant and wound\u2010healing activities. In this study, aqueous (AW) and aqueous ethanolic (AE) extracts obtained from the aerial parts of\n                    <jats:italic>Anchusa azurea<\/jats:italic>\n                    by ultrasonic extraction were evaluated for their phytochemical composition, antioxidant activity, cytotoxicity, and wound\u2010healing efficacy using in vitro and in vivo models. The AE extract exhibited higher total phenolic and flavonoid contents and stronger antioxidant activity than the AW extract. Cytotoxicity was assessed on human skin fibroblasts, adult (HDFa) cells, using the MTT assay. Both extracts showed no significant cytotoxic effects. AW\u2010loaded nanoliposomes (AWNP) and AE\u2010loaded nanoliposomes (AENP) were successfully prepared, and scanning electron microscopy, transmission electron microscopy, and dynamic light scattering analyses confirmed nanoscale vesicles with negative zeta potential values. In vitro release studies demonstrated biphasic release profiles, with AENP exhibiting greater cumulative release than AWNP. In a rat excisional wound model, nanoliposomal formulations significantly enhanced wound contraction compared with crude extracts and controls. Histopathological evaluation demonstrated increased collagen deposition, neovascularization, and re\u2010epithelialization, particularly in the AE and AENP groups. These findings suggest that the phenolic\u2010rich aqueous ethanolic extract and its nanoliposomal formulation are promising candidates for wound\u2010healing applications.\n                  <\/jats:p>","DOI":"10.1002\/open.70254","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:02:20Z","timestamp":1786680140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Wound\u2010Healing Properties of\n                    <i>Anchusa azurea<\/i>\n                    : A Nanoliposome\u2010Based Approach"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4319-7558","authenticated-orcid":false,"given":"Sevim Feyza","family":"Erdo\u011fmu\u015f","sequence":"first","affiliation":[{"name":"Department of Basic Pharmaceutical Sciences Faculty of Pharmacy Afyonkarahisar Health Sciences University  Afyonkarahisar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan H\u00fcseyin","family":"Demirel","sequence":"additional","affiliation":[{"name":"Bayat Vocational School Department of Laboratory and Veterinary Health Afyon Kocatepe University  Afyonkarahisar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"Erdal Alt\u0131nta\u015f","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics Faculty of Health Sciences Afyonkarahisar Health Sciences University  Afyonkarahisar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130ffet \u0130rem","family":"Tatl\u0131 \u00c7ankaya","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany Faculty of Pharmacy Hacettepe University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurullah","family":"Okumu\u015f","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Pediatrics Afyonkarahisar Health Sciences University  Afyonkarahisar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000225430.42531.c2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00067.2017"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2647"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806\u20104841.20164741"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.10.032"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970\u20100358.101321"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.07.038"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2013.0506"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2684108"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18051061"},{"issue":"2","key":"e_1_2_11_12_1","first-page":"177","article-title":"Phenolic Compounds from the Roots of Anchusa Azurea Var. 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