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The structures were supported by FT\u2010IR, NMR, and APCI\u2010HRMS analyses. The compounds were evaluated against clinically relevant bacterial and fungal strains, complemented by antioxidant assays, hemocompatibility\/antihemolysis models, cancer cell viability assays, and in silico drug\u2010likeness, pharmacokinetic, pharmacogenomic, and toxicity prediction. The biological profile was substituent\u2010dependent:\n                    <jats:bold>1b<\/jats:bold>\n                    showed the strongest activity against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MIC\u2009=\u20090.25\u2009mg\/mL),\n                    <jats:bold>1a<\/jats:bold>\n                    and\n                    <jats:bold>1c<\/jats:bold>\n                    were the most active against\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    (MIC\u2009=\u20090.31\u2009mg\/mL), while the iodophenyl derivative\n                    <jats:bold>1d<\/jats:bold>\n                    was the most potent against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    (MIC\u2009=\u20090.16\u2009mg\/mL). Compounds\n                    <jats:bold>1b<\/jats:bold>\n                    and\n                    <jats:bold>1c<\/jats:bold>\n                    also inhibited microbial adherence in selected Gram\u2010positive and fungal models. Compound\n                    <jats:bold>1a<\/jats:bold>\n                    displayed the most consistent antioxidant activity across antioxidant assays and protected erythrocytes from AAPH\u2010induced oxidative hemolysis. The cytotoxicity screening revealed increased sensitivity of A\u2010431 cells, particularly to\n                    <jats:bold>1b<\/jats:bold>\n                    (IC50\u2009=\u200913.67\u2009\u00b5g\/mL). Molecular docking revealed key binding interactions potentially underlying the observed biological activity. Overall, the integrated data identify carbazole\u2013acylthiourea propanamides, especially\n                    <jats:bold>1a<\/jats:bold>\n                    and\n                    <jats:bold>1b<\/jats:bold>\n                    , as promising bioactive scaffolds for further optimization as multifunctional compounds.\n                  <\/jats:p>","DOI":"10.1002\/open.70258","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:26:23Z","timestamp":1791822383000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Carbazole\u2013Acylthiourea Derivatives: Synthesis, Antimicrobial, Antioxidant, Hemocompatibility, Cytotoxicity, and ADME\u2010Tox Profiling"],"prefix":"10.1002","volume":"15","author":[{"given":"Carmen","family":"Limban","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy \u201cCarol Davila\u201d University of Medicine and Pharmacy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana Cristina","family":"Marina\u015f","sequence":"additional","affiliation":[{"name":"Research Institute of the University of Bucharest (ICUB) University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Carmen","family":"Chifiriuc","sequence":"additional","affiliation":[{"name":"Research Institute of the University of Bucharest (ICUB) University of Bucharest  Bucharest Romania"},{"name":"Department of Microbiology Faculty of Biology University of Bucharest  Bucharest Romania"},{"name":"Romanian Academy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miron Teodor","family":"C\u0103proiu","sequence":"additional","affiliation":[{"name":"\u201cC.D. Nenitzescu\u201d Institute of Organic and Supramolecular Chemistry of the Romanian Academy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florea","family":"Dumitra\u015fcu","sequence":"additional","affiliation":[{"name":"\u201cC.D. Nenitzescu\u201d Institute of Organic and Supramolecular Chemistry of the Romanian Academy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erzs\u00e9bet\u2010Eleon\u00f3ra","family":"Kapronczai","sequence":"additional","affiliation":[{"name":"Supramolecular Organic and Organometallic Chemistry Centre Department of Chemistry Faculty of Chemistry and Chemical Engineering Babe\u015f\u2010Bolyai University  Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Zarafu","sequence":"additional","affiliation":[{"name":"Research Institute of the University of Bucharest (ICUB) University of Bucharest  Bucharest Romania"},{"name":"Department of Organic Chemistry, Biochemistry and Catalysis Faculty of Chemistry University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Camelia","family":"Nu\u0163\u0103","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy \u201cCarol Davila\u201d University of Medicine and Pharmacy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petre","family":"Ioni\u0163\u0103","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Biochemistry and Catalysis Faculty of Chemistry University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Speran\u0163a","family":"Avram","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Physiology, and Biophysics Faculty of Biology University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u0103t\u0103lina","family":"Mare\u015f","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Physiology, and Biophysics Faculty of Biology University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andra Maria","family":"P\u0103un","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Physiology, and Biophysics Faculty of Biology University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan Mihai","family":"Cristea","sequence":"additional","affiliation":[{"name":"Preclinical Department 2 Morphological Sciences Faculty of Medicine Carol Davila University of Medicine and Pharmacy Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmellina Daniela","family":"B\u0103diceanu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy \u201cCarol Davila\u201d University of Medicine and Pharmacy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilinca Margareta","family":"Vlad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy \u201cCarol Davila\u201d University of Medicine and Pharmacy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Coand\u0103","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy \u201cCarol Davila\u201d University of Medicine and Pharmacy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delia Laura","family":"Popescu","sequence":"additional","affiliation":[{"name":"Faculty of Interdisciplinary Studies University of Bucharest  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralia","family":"Bleotu","sequence":"additional","affiliation":[{"name":"Research Institute of the University of Bucharest (ICUB) University of Bucharest  Bucharest Romania"},{"name":"Department of Cellular and Molecular Pathology \u015etefan S. 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