{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:26:01Z","timestamp":1786940761218,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    In this study, the targeted phytochemical profiles and multi\u2010target biological activities of\n                    <jats:italic>Mandragora autumnalis<\/jats:italic>\n                    L. and\n                    <jats:italic>Spartium junceum<\/jats:italic>\n                    L. were evaluated using liquid chromatography\u2013tandem mass spectrometry (LC\u2010MS\/MS), antioxidant, enzyme inhibition, and antimicrobial assays. LC\u2010MS\/MS analysis showed that\n                    <jats:italic>M. autumnalis<\/jats:italic>\n                    predominantly contained low\u2010molecular\u2010weight phenolic acids, particularly protocatechuic acid (0.6850\u2009\u00b5g\/mg), whereas\n                    <jats:italic>S. junceum<\/jats:italic>\n                    was richer in flavonoids and isoflavonoids, especially genistein (1.8280\u2009\u00b5g\/mg) and cynaroside (1.5240\u2009\u00b5g\/mg).\n                    <jats:italic>M. autumnalis<\/jats:italic>\n                    showed stronger antioxidant activity, with a DPPH IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 60.16 \u00b5g\/mL and a FRAP absorbance of 2.098\u2009\u00b1\u20090.042 at 45 \u00b5g\/mL, whereas\n                    <jats:italic>S. junceum<\/jats:italic>\n                    showed weak DPPH radical scavenging activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 813.50\u2009\u00b5g\/mL).\n                    <jats:italic>M. autumnalis<\/jats:italic>\n                    also exhibited stronger acetylcholinesterase and carbonic anhydrase inhibition, on the other hand\n                    <jats:italic>S. junceum<\/jats:italic>\n                    displayed a butyrylcholinesterase\u2010oriented inhibitory profile. Preliminary antimicrobial screening revealed inhibition zones of 11\u201313\u2009mm against selected bacterial strains. Overall, the findings suggest that low\u2010molecular\u2010weight phenolic acids may contribute to the biological activity of\n                    <jats:italic>M. autumnalis<\/jats:italic>\n                    , whereas flavonoids and isoflavonoids may support the characteristic inhibitory profile of\n                    <jats:italic>S. junceum<\/jats:italic>\n                    , highlighting the importance of qualitative metabolite composition in shaping extract bioactivity.\n                  <\/jats:p>","DOI":"10.1002\/open.70269","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T07:27:07Z","timestamp":1786606027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolite\u2010Driven Biological Activity of\n                    <i>Mandragora autumnalis<\/i>\n                    and\n                    <i>Spartium junceum<\/i>\n                    : A Comparative LC\u2010MS\/MS Study on Antioxidant, Cholinesterase, and Carbonic Anhydrase Inhibition"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8388-5470","authenticated-orcid":false,"given":"Kubra","family":"Aslan","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Science Ataturk University  Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esra","family":"Elbir","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science Ataturk University  Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Bicakci","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Ataturk University  Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silanur","family":"Ozdogan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science Ataturk University  Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4090-7227","authenticated-orcid":false,"given":"Mustafa Abdullah","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy Dicle University  Diyarbakir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"Ilhami","family":"Gulcin","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Ataturk University  Erzurum T\u00fcrkiye"},{"name":"Rectorate of Agri Ibrahim Cecen University  Agri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202403588"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17010050"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jemermed.2017.04.033"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2018.01.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph18030328"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26178506"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/open.70227"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109258(18)95800\u2010X"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2012.04.044"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202504032"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750\u20103841.2011.02142.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/life13030735"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2020.112347"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2025.2481148"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010011\u20100774\u20102"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2008.05.003"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","unstructured":"M.Aksoy I.Gulcin andO. 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