{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:04:19Z","timestamp":1789981459725,"version":"4.0.1"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistryOpen"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>\n                    In this study, development and optimization of a multiplex PCR assay for the simultaneous amplification of selected bacterial and fungal targets were performed. Source of hospital\u2010acquired infections\u2010specific, (\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    ,\n                    <jats:italic>Fusarium oxysporum<\/jats:italic>\n                    , and\n                    <jats:italic>Scedosporium prolificans<\/jats:italic>\n                    ) primer sets targeting conserved genomic regions were systematically evaluated for potential nonspecific interactions, cross\u2010reactivity, and structural stability using in silico tools and empirical analyses. Multiplex PCR conditions were optimized using genomic DNA from reference strains under controlled laboratory conditions, with particular emphasis on annealing temperature, MgCl\n                    <jats:sub>2<\/jats:sub>\n                    concentration, and primer balance. In addition, the effect of gold nanoparticles (AuNPs) on amplification performance was investigated. The inclusion of 1.0\u2009nM 10\u2009nm AuNPs enhanced overall amplification efficiency, with a more pronounced improvement observed for fungal targets. Overall, this study establishes an analytical proof of concept for a nanoparticle\u2010assisted multiplex PCR configuration capable of simultaneously amplifying multiple bacterial and fungal targets in a single reaction.\n                  <\/jats:p>","DOI":"10.1002\/open.70294","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:06:33Z","timestamp":1789535193000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Gold Nanoparticle\u2010Assisted Multiplex Polymerase Chain Reaction Assay for Selected Hospital\u2010Acquired Infections\u2010Associated Bacterial and Fungal Pathogens"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8388-5470","authenticated-orcid":false,"given":"Kubra","family":"Aslan","sequence":"first","affiliation":[{"name":"Chemistry Department Faculty of Science Ataturk University  Yakutiye Erzurum 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