{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:51:10Z","timestamp":1786978270653,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T00:00:00Z","timestamp":1707091200000},"content-version":"vor","delay-in-days":35,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["OTO Open"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Sinus disease is prevalent in persons with cystic fibrosis (PwCF) and may be a reservoir of airway infection in postlung transplant (pTx) patients. The microbial composition of cystic fibrosis sinuses and its associations with chronic rhinosinusitis (CRS) is relatively unexplored. We aimed to examine the sinus and lower airway microbiome and their associations with CRS in PwCF and pTxPwCF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Prospective single\u2010centre study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>A total of 31 sex and age (\u00b12 years) matched PwCF and pTxPwCF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Demographic and clinical data along with sinus swabs and sputum were collected. CRS was assessed using Sinonasal Outcome Test\u201022 (SNOT\u201022) (patient reported outcome) and Lund\u2010McKay (computed tomography sinus) scores. Samples underwent MiSeq Illumina sequencing of the universal 16S ribosomal RNA gene.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 31 PwCF (15 pTxPwCF) were included. Aggregate airways microbiome composition was dominated by\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      (46%),\n                      <jats:italic>Haemophilus<\/jats:italic>\n                      (14%),\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      (11%),\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      (10%), and\n                      <jats:italic>Fusobacterium<\/jats:italic>\n                      (6%). \u03b1\u2010diversity was significantly lower in post\u2010Tx samples across both sputum and sinus samples (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.005). \u03b2\u2010diversity was significantly different between sputum (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.004), but not sinus (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.75) samples by transplant status. While there was a trend in higher \u03b2\u2010diversity associated with lower SNOT\u201022 score at time of first visit, this did not reach significance (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Sinus and airway microbiomes differed in PwCF and pTxPwCF, but the prevalent organisms remained consistent. Elucidating the relationship of the microbiome with clinical status to better understand when to intervene accordingly is needed to optimize sinus disease management in PwCF.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/oto2.101","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T03:25:25Z","timestamp":1707276325000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluating Sinus Microbiology by Transplant Status in Persons With Cystic Fibrosis: A Matched Cohort Study"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3731-9675","authenticated-orcid":false,"given":"Ranjani","family":"Somayaji","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine University of Calgary Calgary Canada"},{"name":"Department of Medicine, Cumming School of Medicine, Alberta Health Services University of Calgary  Calgary Canada"},{"name":"Snyder Institute for Chronic Diseases University of Calgary  Calgary Canada"},{"name":"Department of Community Health Sciences, Cumming School of Medicine University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina S.","family":"Thornton","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine University of Calgary Calgary Canada"},{"name":"Department of Medicine, Cumming School of Medicine, Alberta Health Services University of Calgary  Calgary Canada"},{"name":"Snyder Institute for Chronic Diseases University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Acosta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine University of Calgary Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck surgery University of Utah  Salt Lake City Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Surgery, Otolaryngology\u2013Head and Neck Surgery, Cumming School of Medicine University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Fatovich","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cumming School of Medicine, Alberta Health Services University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitesh V.","family":"Thakrar","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cumming School of Medicine, Alberta Health Services University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Parkins","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine University of Calgary Calgary Canada"},{"name":"Department of Medicine, Cumming School of Medicine, Alberta Health Services University of Calgary  Calgary Canada"},{"name":"Snyder Institute for Chronic Diseases University of Calgary  Calgary 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