{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:39:33Z","timestamp":1792647573021,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020485","name":"CooperVision","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020485","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["OVS"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The purpose of this study was to investigate the quantity of radioactive lysozyme deposited on various rigid corneal lenses, and the impact of using a care solution containing a surfactant.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seven rigid corneal lens materials were investigated in this study. Lenses were soaked in an artificial tear solution (ATS), containing, among other components, radioactively labelled\n                      <jats:sup>125<\/jats:sup>\n                      I\u2010lysozyme, for 18\u00a0h at 37\u00b0C, followed by a soak for 6\u00a0h in saline. Alternatively, a one\u2010step peroxide\u2010based disinfection solution, which contains a poloxamer as a surfactant, was used in place of saline to determine the impact of use of a care solution on deposition. This process of soaking in the ATS followed by soaking in saline or the care solution was repeated for 30 cycles to simulate one month of lens wear. The amount of radioactive lysozyme remaining on the different lenses after 30 cycles was then quantified through gamma radiation counts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Use of radioactively labelled\n                      <jats:sup>125<\/jats:sup>\n                      I\u2010lysozyme allowed for quantification of protein down to 0.19\u00a0\u03bcg\/lens. Five of the seven contact lens materials tested showed a significant reduction in the amount of radioactive lysozyme remaining after using the care solution (range 1.33\u00a0\u00b1\u00a00.11\u20132.47\u00a0\u00b1\u00a00.13\u00a0\u03bcg\/lens) compared to if only saline was used (range 1.25\u00a0\u00b1\u00a00.18\u20133.21\u00a0\u00b1\u00a00.66\u00a0\u03bcg\/lens) after 30 simulated cycles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The small amounts of lysozyme deposited on rigid contact lenses can be quantified using radioactively labelled\n                      <jats:sup>125<\/jats:sup>\n                      I\u2010lysozyme. Use of a care solution leads to lower amounts of lysozyme remaining on the lenses compared to saline, however, some lysozyme still remains and is not removed.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ovs2.70108","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:36:36Z","timestamp":1791797796000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Radioactive lysozyme deposition on rigid corneal lenses with or without use of a contact lens care solution"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4151-0384","authenticated-orcid":false,"given":"Alex","family":"Hui","sequence":"first","affiliation":[{"name":"School of Optometry and Vision Science Faculty of Medicine and Health UNSW Sydney  Sydney New South Wales Australia"},{"name":"School of Optometry &amp; Vision Science University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3139-0422","authenticated-orcid":false,"given":"Miriam","family":"Heynen","sequence":"additional","affiliation":[{"name":"Centre for Ocular Research &amp; Education University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2849-911X","authenticated-orcid":false,"given":"Parisa","family":"Mirzapour","sequence":"additional","affiliation":[{"name":"Centre for Ocular Research &amp; Education University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daryl","family":"Enstone","sequence":"additional","affiliation":[{"name":"Centre for Ocular Research &amp; Education University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-8428","authenticated-orcid":false,"given":"Melanie","family":"George","sequence":"additional","affiliation":[{"name":"CooperVision  San Ramon California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3324-1065","authenticated-orcid":false,"given":"William","family":"Ngo","sequence":"additional","affiliation":[{"name":"School of Optometry &amp; Vision Science University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7409-7349","authenticated-orcid":false,"given":"Lyndon","family":"Jones","sequence":"additional","affiliation":[{"name":"School of Optometry &amp; Vision Science University of Waterloo  Waterloo Ontario Canada"},{"name":"Centre for Ocular Research &amp; Education University of Waterloo  Waterloo Ontario Canada"},{"name":"Centre for Eye and Vision Research (CEVR)  Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/opx.0000000000000615"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clae.2014.11.110"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/opx.0b013e3180485d4b"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clae.2008.05.004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clae.2011.12.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clae.2012.01.003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.013"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/icl.0b013e318275305b"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/opx.0b013e318292bb13"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2020.03.007"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/icl.0b013e318272c470"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.32927"},{"key":"e_1_2_8_14_1","first-page":"138","article-title":"Disinfection associated spoilage of high water content ionic matrix hydrogels","volume":"15","author":"Sack RA","year":"1989","journal-title":"CLAO J"},{"key":"e_1_2_8_15_1","first-page":"842","article-title":"Specificity and biological activity of the protein deposited on the hydrogel surface. 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