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To investigate activation of effector caspases <jats:italic>in vivo<\/jats:italic> in biopsy specimens of lymphomas, a new assay was developed using antibodies against active caspase 3 and p89, a protein fragment generated by caspase\u2010specific cleavage of poly\u2010ADP ribose polymerase (PARP). Using this assay, it was found that in B\u2010cell lymphomas, levels of active caspase 3\/p89\u2010positive cells correlate strongly with morphologically recognizable apoptotic cells. The number of active caspase 3\/p89\u2010positive cells was low in follicular lymphomas and usually high in diffuse large cell lymphomas. Highest numbers were found in Burkitt lymphomas and in two biopsies of diffuse large B\u2010cell lymphomas (DLCLs) obtained several days after initiation of therapy. It is concluded that apoptosis in reactive lymphoid tissues and in B\u2010cell lymphomas always involves activation of effector caspase 3 and cleavage of one of the major effector caspase substrates, PARP\u20101. Moreover, levels of effector caspase activation are constantly low in low\u2010grade follicular lymphomas and vary considerably in DLCL and Burkitt lymphoma. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1046","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T07:25:48Z","timestamp":1033889148000},"page":"307-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Apoptosis in B\u2010cell lymphomas and reactive lymphoid tissues always involves activation of caspase 3 as determined by a new <i>in situ<\/i> detection method"],"prefix":"10.1002","volume":"196","author":[{"given":"Danny F.","family":"Dukers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joost J.","family":"Oudejans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wim","family":"Vos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosita L.","family":"ten Berge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris J. 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