{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T08:27:20Z","timestamp":1765700840737,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,1,22]],"date-time":"2002-01-22T00:00:00Z","timestamp":1011657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Following an earlier study linking monosomy 9 with recurrence of transitional cell carcinomas (TCCs) of the urinary bladder, 109 primary and recurrent TCCs (from 47 patients) were examined to explore genetic alterations at chromosome 9 associated with recurrence. Patient DNA was microdissected and extracted from archival tissue sections and analysed for loss of heterozygosity (LOH) at three regions on chromosome 9 where tumour suppressor genes (TSGs) are known to reside (<jats:italic>INK 4A<\/jats:italic>, <jats:italic>DBC1<\/jats:italic>, and <jats:italic>TSC1<\/jats:italic>). Patients were categorized into two groups, non\u2010recurrent TCC (NR, <jats:italic>n<\/jats:italic>=18) and recurrent TCC (REC, <jats:italic>n<\/jats:italic>=29). It was noted that 12% of NR tumours, compared with 54% of REC primary tumours (<jats:italic>p<\/jats:italic>=0.01), had LOH at all informative markers spanning the <jats:italic>TSC1<\/jats:italic> region. The risk of recurrence was significantly higher in patients with deleted <jats:italic>TSC1<\/jats:italic> than in those who retained the <jats:italic>TSC1<\/jats:italic> region (<jats:italic>p<\/jats:italic>=0.035). Levels of LOH at <jats:italic>DBC1<\/jats:italic> or <jats:italic>INK 4A<\/jats:italic> were not significantly different in NR tumours than in REC primary tumours and recurrence\u2010free survival was not affected by loss of either of these genes. Loss of all informative markers spanning chromosome 9 was observed in 0% of NR tumours compared with 25% of REC primary tumours (<jats:italic>p<\/jats:italic>=0.04). The probability of recurrence was also significantly increased in patients who had LOH at all informative markers spanning chromosome 9 (<jats:italic>p<\/jats:italic>=0.016), confirming earlier fluorescence <jats:italic>in situ<\/jats:italic> hybridization results. This study provides further evidence that recurrence in bladder cancer is a distinct event, with underlying molecular causes. It also identifies the <jats:italic>TSC1<\/jats:italic> locus as a candidate for a TSG, which drives recurrence in a proportion of TCC patients. Loss of all informative markers, including those residing in the <jats:italic>TSC1<\/jats:italic> region, spanning chromosome 9 was also linked to recurrence. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1052","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:00:41Z","timestamp":1033891241000},"page":"380-385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Identification of loci associated with putative recurrence genes in transitional cell carcinoma of the urinary bladder"],"prefix":"10.1002","volume":"196","author":[{"given":"Joanne","family":"Edwards","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Duncan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J.","family":"Going","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda D.","family":"Watters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth M.","family":"Grigor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M. 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