{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:19:35Z","timestamp":1790878775911,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,1,22]],"date-time":"2002-01-22T00:00:00Z","timestamp":1011657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b2\u2010galactosidase activity at pH\u20096 is associated <jats:italic>in vitro<\/jats:italic> with senescence and cellular death, but <jats:italic>in vivo<\/jats:italic> data are sparse. This study undertook firstly to map \u2018senescence\u2010associated\u2019 \u03b2\u2010galactosidase activity (SA\u03b2G) at pH\u20096 in normal epithelia and mucosae of the upper gastrointestinal tract. As escape from senescence confers a proliferative advantage, a reduction in SA\u03b2G activity might be predicted in neoplasia and their precursors <jats:italic>in vivo<\/jats:italic>. This prediction was tested in metaplastic, dysplastic, and neoplastic epithelium of the upper gastrointestinal tract. Histochemical staining for SA\u03b2G was performed at pH\u20096 on cryostat sections of 350 endoscopic biopsies from sites including oesophagus, stomach, and duodenum of 46 patients: 28 with Barrett's oesophagus (two with adenocarcinoma), 15 with gastric adenocarcinoma, and three with oesophageal squamous cancer. A staining score (range 0\u20136) was assigned to epithelial cells in all mucosae and scores were calculated for surface (luminal), intermediate, and deep (basal) layers. The strongest SA\u03b2G activity was in surface luminal cells of normal duodenal mucosa (mean score 3.6\u00b10.5; <jats:italic>n<\/jats:italic>=19), \u2018specialized\u2019 Barrett's mucosa (mean 2.2\u00b10.12; <jats:italic>n<\/jats:italic>=105), and intestinal metaplasia in the stomach (mean 2.4\u00b10.40; <jats:italic>n<\/jats:italic>=16). Squamous epithelium was consistently negative for SA\u03b2G activity. Low\u2010 and high\u2010grade Barrett's dysplasia showed no decrease in SA\u03b2G activity, but reduced activity was seen in gastric and oesophageal adenocarcinomas (mean 1.24\u00b10.29; <jats:italic>n<\/jats:italic>=17; <jats:italic>p<\/jats:italic>=0.012). In six gastric adenocarcinomas, there was no detectable activity. Whether SA\u03b2G is truly a marker of cellular senescence <jats:italic>in vivo<\/jats:italic> remains to be determined. Activity is low in mucosal proliferation compartments and increases with cellular differentiation, especially in native or metaplastic intestinal mucosae. SA\u03b2G activity persists in dysplastic mucosae but may show some reduction or loss in adenocarcinomas (<jats:italic>p<\/jats:italic>=0.0012). Loss of SA\u03b2G activity is not, therefore, an early event in glandular dysplasia\u2013neoplasia of the upper gastrointestinal tract. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1059","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:00:41Z","timestamp":1033891241000},"page":"394-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["\u2018Senescence\u2010associated\u2019 \u03b2\u2010galactosidase activity in the upper gastrointestinal tract"],"prefix":"10.1002","volume":"196","author":[{"given":"James J.","family":"Going","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Stuart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Downie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aileen 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